Nonhuman primate scientific research support component

非人灵长类支持组件的目标是通过在体内进行被动保护或增强研究来表征CHAVI-ID研究人员产生的单抗的功能活性，通过使用CHAVI研究人员开发的新免疫原进行免疫研究，以及进行相关的单抗和免疫功能研究以恒河猴为模型来加速HLV-1疫苗的开发，从而支持CHAVI-ID的整体计划。我们在许多补充领域取得的最新进展将促进这项工作，包括开发新型粘膜SIV/SIV挑战模型、表达已传递/创建者HLV-1包膜的新型SIV构建体、显著提高HLV-1包膜蛋白免疫原免疫原性的佐剂，以及从免疫的恒河猴中产生单抗的重组技术。 具体目标 目的1.对恒河猴进行被动保护研究，以确定具有明确特异性的抗体和CD8 T细胞产生的因子在阻断SIV/SIV粘膜获取方面的有效性。 目的2.在恒河猴身上进行免疫研究，探索疫苗诱导抗体的新策略，这些抗体可能会预防HIV-1感染。 目的3.探讨免疫恒河猴体内广谱中和抗HIV-1抗体的演变。

The goal of the Nonhuman Primate Support Component is to support the overall program of the CHAVI-ID by performing in vivo passive protection or enhancement studies to characterize the functional activities of mAbs generated by CHAVI-ID investigators, by performing immunization studies with novel immunogens developed by CHAVI investigators, and performing correlative mAb and immune functional studies to use the rhesus monkey as a model to accelerate HlV-1 vaccine development. This work will be facilitated by recent advances we have made in a number of complementing areas, including the development of novel mucosal SIV/SHIV challenge models, novel SHIV constructs expressing transmitted/founder HlV-1 envelopes, adjuvants that substantially increase the immunogenicity of HlV-1 envelope protein immunogens, and recombinant technology for generating monoclonal antibodies from immunized rhesus macaques. Specific Aims Aim 1. Pursue passive protection studies in rhesus macaques to determine the effectiveness of antibodies with defined specificities and CD8 T cell-produced factors in blocking SHIV/SIV mucosal acquisition. Aim 2. Perform immunization studies in rhesus macaques to explore novel strategies for vaccine induction of antibodies that might protect against HIV-1 acquisition. Aim 3. Explore the evolution of broadly neutralizing anti-HIV-1 antibodies in vaccinated rhesus macaques.