Synthetic Antigen Binder Core

合成抗原结合剂核心

• 批准号: 9351543
• 负责人: ANTHONY A KOSSIAKOFF
• 金额: $ 17.85万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-10 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9351543
• 关键词: Address; Affinity; Characteristics; Collaborations; Communities; Community Outreach; Complex; Crystallization; Crystallography; Custom; Detergents; Engineering; Environment; Epitopes; Fruit; Generations; Goals; Lipids; Location; Membrane Proteins; Methodology; Modification; Molecular Chaperones; Molecular Conformation; Outcome; Performance; Play; Population; Property; Protein Conformation; Proteins; Reagent; Research; Research Infrastructure; Services; Shapes; Site; Specificity; Structural Protein; Structure; Synthetic Antigens; System; Technology; Untranslated RNA; Work; channel blockers; complex IV; design; improved; inhibitor/antagonist; member; nanodisk; non-Native; novel; protein complex; protein function; scaffold; synthetic antibodies; tool

The overarching goal of the Synthetic Antibody Binder Sab Core is to develop a broad range of powerful approaches and provide novel reagents to support the consortium¹s and community¹s research objectives. The Sab Core is uniquely capable to generate in a high-throughput way synthetic antibodies (Sabs) to an extensive range of targets including soluble proteins, protein complexes and membrane proteins. Thus, the infrastructure in place with extensive capability to efficiently generate high-quality affinity reagents that will dramatically accelerate membrane protein research performed within the MPSDC Consortium, as well as in the entire membrane protein research community. The goals of the Sab Core are (i) to provide high-quality synthetic affinity reagents for membrane protein targets using state-of-the-art technologies, (ii) to accelerate structure determination by Sab-assisted crystallography and Cryo-EM and (iii) to develop novel applications of Sabs that will enable Core users to significantly elevate the level of mechanistic understanding of membrane protein functions.

合成抗体结合剂SAB核心的首要目标是开发一种广泛的 一系列强有力的方法和提供新的试剂来支持财团？S 和社区？S的研究目标。SAB Core独一无二地能够在 针对广泛靶点的高通量合成抗体(Sabs) 包括可溶性蛋白质、蛋白质复合体和膜蛋白。因此， 基础设施到位，具有广泛的能力，可高效地产生高质量的亲和力 将极大地加速膜蛋白研究的试剂 MPSDC联盟，以及整个膜蛋白研究社区。 SAB核心的目标是(I)为以下方面提供高质量的合成亲和试剂 使用最先进技术的膜蛋白靶标，(Ii)加速结构 SAB辅助结晶学和低温电子显微镜的测定及(III)开发新的 SABS的应用将使核心用户能够显著提升 从机理上理解膜蛋白的功能。