ShEEP Request for Super Resolution Laser Scanning Confocal Microscopy System

ShEEP 请求超分辨率激光扫描共焦显微镜系统

• 批准号: 9361137
• 负责人: ROLANDO E RUMBAUT
• 金额: --
• 依托单位: MICHAEL E DEBAKEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-01-01 至 2017-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9361137
• 关键词: Biological; Blood Coagulation Disorders; Blood Platelets; Blood coagulation; Caliber; Carbon Dioxide; Cell Culture Techniques; Cells; Chronic Kidney Failure; Collaborations; Commuting; Complement; Computer Hardware; Computer software; Computers; Core Facility; Cultured Cells; Diabetes Mellitus; Disease; Electron Microscopy; Equipment; Event; Face; Fluorescent Dyes; Fostering; Goals; Humidity; Hybrids; Image; Individual; Laser Scanning Confocal Microscopy; Lasers; Lead; Medical center; Microscope; Microscopy; Modeling; Operating System; Program Evaluation; Recruitment Activity; Research; Research Personnel; Resolution; Sampling; Scanning; Schedule; Scientist; Shapes; Silicone Oils; Site; Speed; Structure; System; Talents; Technology; Temperature; Time; Tissues; Training; Veterans; cellular imaging; cellular targeting; design; flasks; improved; instrument; light microscopy; microscopic imaging; nanometer; optical spectra; programs; solid state; vibration

This application will support the purchase of a state-of-the-art microscopy system, a super resolution laser scanning confocal microscope (Olympus FV3000). This system will enhance the research program of 7 VA investigators and enable them to obtain microscopy images of cellular and subcellular events with unprecedented resolution (up to 120 nanometers). There is no equipment available on our campus for this purpose. Although our academic affiliate has comparable microscopy systems in core facilities, our VA investigators face obstacles in using them due to limited availability in scheduling which often lead to significant delays, and cumbersome commute to/from our affiliate with biological samples. Having this technology available on our campus will represent a major benefit to our VA researchers, providing exceptional microscopy capabilities. For example, 4 of the users study platelets (cells with diameter of 2-3 micrometers) and typically rely on electron microscopy to study their cellular components. The super resolution system will enable these researchers to obtain microscopy images of platelet shape and structure with unprecedented capabilities. The system contains 7 lasers, which will facilitate simultaneous imaging of multiple cellular targets, including identify their interactions. Further, the system will be particularly helpful for studies on cultured cells, with its inverted microscope design with high numerical aperture silicone oil objectives and a temperature, humidity, and carbon dioxide-controlled incubation chamber. This system will complement the existing core research equipment at our facility, and will provide a tremendous enhancement to our overall research program. This is expected to promote further collaboration among VA investigators as well as improve our efforts to recruit and retain talented investigators to our medical center.

此应用程序将支持购买最先进的显微镜