ShEEP Request for Super Resolution Laser Scanning Confocal Microscopy System

这个应用程序将支持购买最先进的显微镜 系统采用超分辨率激光扫描共聚焦显微镜(奥林巴斯FV3000)。这 系统将增强7名退伍军人管理局调查人员的研究计划，使他们能够 获得史无前例的细胞和亚细胞事件的显微图像 分辨率(最高120纳米)。我们的校园里没有设备可以用来 这就是目的。尽管我们的学术分支机构有类似的显微镜系统 核心设施，我们的退伍军人管理局调查人员在使用这些设施时面临障碍，原因是 经常导致重大延迟和繁琐的调度的可用性 携带生物样本往返于我们的分支机构。有了这项技术 对我们的退伍军人研究人员来说，这将是一个重大的好处，提供 超凡的显微镜能力。例如，4的用户研究血小板(细胞 直径为2-3微米)，通常依靠电子显微镜来研究 它们的细胞成分。超分辨率系统将使这些研究人员能够 获得史无前例的血小板形状和结构的显微图像 能力。该系统包含7个激光，这将有助于同时成像 多个细胞靶标，包括识别它们的相互作用。此外，该系统将是 对培养细胞的研究特别有帮助，其倒置显微镜设计具有 高数值孔径硅油物镜和温度、湿度和碳 二氧化碳控制的孵化室。这一系统将补充现有的核心 研究设备，并将极大地增强我们的 总体研究计划。预计这将促进退伍军人管理局之间的进一步合作 以及加强我们招募和留住有才华的调查人员的努力 我们的医疗中心。

This application will support the purchase of a state-of-the-art microscopy system, a super resolution laser scanning confocal microscope (Olympus FV3000). This system will enhance the research program of 7 VA investigators and enable them to obtain microscopy images of cellular and subcellular events with unprecedented resolution (up to 120 nanometers). There is no equipment available on our campus for this purpose. Although our academic affiliate has comparable microscopy systems in core facilities, our VA investigators face obstacles in using them due to limited availability in scheduling which often lead to significant delays, and cumbersome commute to/from our affiliate with biological samples. Having this technology available on our campus will represent a major benefit to our VA researchers, providing exceptional microscopy capabilities. For example, 4 of the users study platelets (cells with diameter of 2-3 micrometers) and typically rely on electron microscopy to study their cellular components. The super resolution system will enable these researchers to obtain microscopy images of platelet shape and structure with unprecedented capabilities. The system contains 7 lasers, which will facilitate simultaneous imaging of multiple cellular targets, including identify their interactions. Further, the system will be particularly helpful for studies on cultured cells, with its inverted microscope design with high numerical aperture silicone oil objectives and a temperature, humidity, and carbon dioxide-controlled incubation chamber. This system will complement the existing core research equipment at our facility, and will provide a tremendous enhancement to our overall research program. This is expected to promote further collaboration among VA investigators as well as improve our efforts to recruit and retain talented investigators to our medical center.