Structure/Function Relationships in Cysteine and Cysteamine Dioxygenases

项目摘要 L-半胱氨酸（Cys）是新蛋白质生物合成的重要组成部分， 几种生物学上重要的含硫分子的前体，如辅酶A，牛磺酸， 谷胱甘肽和无机硫酸盐。然而，生物体必须严格调节 外源性半胱氨酸，因为这种半必需氨基酸的水平升高可能是极其有害的。非- 血红素铁酶半胱氨酸双加氧酶（CDO）通过催化半胱氨酸的水解来维持适当的Cys水平。 Cys氧化成半胱氨酸亚磺酸。CDO的功能障碍和随之而来的Cys积累 与几种神经退行性疾病有关 辅酶A合成过程中Cys的脱羧产生半胱胺。本构 辅酶A的降解释放该半胱胺部分，其可以通过酶促转化为亚牛磺酸。 非血红素铁酶半胱胺（2-氨基乙硫醇）双加氧酶（ADO）。亚牛磺酸是 随后氧化为牛磺酸，牛磺酸是一种氨基硫醇酸，在哺乳动物中起着许多重要作用， 组织，包括维持心脏功能，保护神经细胞免受兴奋性毒性和缺血， 作为神经递质，稳定骨骼肌膜。 尽管催化两种结构相似的硫醇化合物的氧化，但CDO和ADO显示出非常高的氧化活性。 底物的低效交叉利用。通过对CDO和ADO的并行研究，提出了一种基于ADO的CDO设计方法， 有机会最终确定每种酶采用的底物选择性机制，从而 Cys和半胱胺水平如何在体内独立调节。的总体目标 因此，本提案中概述的研究旨在确定关键氨基酸残基在以下方面的作用： 底物的选择性，定位和激活的CDO和ADO催化机制。 为此，我们制定了以下具体目标： 1.阐明CDO催化机理中的结构/功能关系。 2.评估真核生物和非真核生物之间关键保守氨基酸残基差异的影响， 原核CDO的活性位点/底物相互作用的性质。 3.确定底物半胱胺和O2与ADO活性位点结合的顺序和模式 以及硫醇氧化的机理。 4.探索CDO和ADO模拟物的几何/电子结构和反应机理。 为了实现这些目标，我们将采用生物化学，光谱和计算相结合的方法。 研究天然酶的静止状态和底物（类似物）加合物的工具，选择 变体和小分子功能性CDO和ADO模拟物。

Project Summary L-Cysteine (Cys) is an essential building block for the biosynthesis of new proteins and serves as a precursor for several biologically important sulfur-containing molecules, such as coenzyme A, taurine, glutathione, and inorganic sulfate. However, organisms must tightly regulate the concentration of exogenous Cys, as elevated levels of this semi-essential amino acid can be extremely harmful. The non- heme iron enzyme cysteine dioxygenase (CDO) serves to maintain the proper Cys levels by catalyzing the oxidation of Cys to cysteine sulfinic acid. Malfunctioning of CDO and the consequent accumulation of Cys have been linked to several neurodegenerative diseases. The decarboxylation of Cys during coenzyme A synthesis generates cysteamine. The constitutive degradation of coenzyme A releases this cysteamine moiety, which can be converted to hypotaurine by the non-heme iron enzyme cysteamine (2-aminoethanethiol) dioxygenase (ADO). Hypotaurine is subsequently oxidized to taurine, an amino thiol acid that plays numerous important roles in mammalian tissues, including maintaining cardiac functions, protecting neural cells from excitotoxicity and ischemia, serving as a neurotransmitter, and stabilizing skeletal muscle membrane. Despite catalyzing the oxidation of two structurally similar thiol compounds, CDO and ADO show very inefficient cross-utilization of substrates. By studying CDO and ADO in parallel, we are presented with an opportunity to conclusively determine the substrate selectivity mechanism each enzyme employs, and thus how Cys and cysteamine levels may be independently regulated in vivo. The overall objective of the research outlined in this proposal is, therefore, to identify the roles of key amino acid residues with regards to substrate selectivity, positioning, and activation in the CDO and ADO catalytic mechanisms. With this objective in mind, we have devised the following Specific Aims: 1. Elucidate structure/function relationships in the catalytic mechanism of CDO. 2. Assess the effects of differences in key conserved amino acid residues between eukaryotic and prokaryotic CDOs on the nature of active site/substrate interactions. 3. Establish the order and modes by which the substrates cysteamine and O2 bind to the ADO active site and the mechanism of thiol oxidation. 4. Explore the geometric/electronic structures and reaction mechanisms of CDO and ADO mimics. To accomplish these aims, we will employ a combination of biochemical, spectroscopic, and computational tools for studying the resting states and substrate (analogue) adducts of the native enzymes, select variants, and small-molecule functional CDO and ADO mimics.