Ribosomally Synthesized Proteins Incorporating Modified Dipeptides

掺入修饰二肽的核糖体合成蛋白质

• 批准号: 9378075
• 负责人: Sidney M. Hecht
• 金额: $ 29.36万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-15 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9378075
• 关键词: Affect; Affinity; Alpha Cell; Amino Acid Motifs; Amino Acids; Antibiotic Resistance; BCL2 gene; Behavior; Binding; Biochemical; Biocompatible Materials; Catalysis; Cell-Free System; Cruciata; Cytosine; DNA; DNA Binding; DNA-Protein Interaction; Dipeptides; Environment; Enzymes; Escherichia coli; Exhibits; Firefly Luciferases; Fluorescence; Goals; Heavy Metals; Helix-Turn-Helix Motifs; Heterogeneous-Nuclear Ribonucleoproteins; In Vitro; Laboratories; Lateral; Light; Luciola; Mediating; Modification; Molecular Conformation; Mono-S; Motion; Nucleic Acid Binding; Nucleic Acids; Organic solvent product; Peptides; Peptidyltransferase; Periodicity; Positioning Attribute; Property; Protein Analysis; Protein Dynamics; Proteins; Publishing; RRM1 gene; Regulatory Element; Research; Resistance; Ribosomes; Role; Site; Techniques; Variant; Work; analog; base; design; flexibility; novel; nucleic acid binding protein; nucleic acid structure; nucleobase; promoter; protein function; protein structure; scaffold; single molecule; synthetic peptide; tool; transcription factor

Project Summary The long term goals of this research involve modification of the peptidyltransferase (PTC) center in bacterial ribosomes to enable the incorporation of very unusual amino acids not recognized by wild- type ribosomes. In recent years, we have described the selection of modified ribosomes and their use in incorporating D-amino acids, beta amino acids, dipeptides and dipeptidomimetric cassettes into proteins. During the five years of proposed research, the focus of efforts will be on the introduction of conformationally constrained cyclic dipeptides and nucleobase amino acids into specific proteins (both as mono- and dipeptides), creating species that have novel properties enabling enhanced analysis of protein function to be realized, as well as the analysis of specific conformational properties essential for enzyme function and strategies for enhancing (and potentially modifying) protein‒DNA recognition.

项目摘要 这项研究的长期目标涉及修饰肽基转移酶（PTC）中心， 细菌核糖体，使非常不寻常的氨基酸不能识别的野生型， 型核糖体。近年来，我们描述了修饰核糖体的选择和它们的用途 在将D-氨基酸、β-氨基酸、二肽和二肽模拟盒掺入 proteins.在拟议的五年研究期间，工作重点将是引进 构象受限的环状二肽和核碱基氨基酸转化为特定的蛋白质 (both如单肽和二肽），创造具有新特性的物种， 分析要实现的蛋白质功能，以及分析特定的构象特性 对酶功能和增强（和潜在修饰）蛋白质DNA的策略至关重要 识别.