Human Antibody Core

人类抗体核心

• 批准号: 9333176
• 负责人: Kenneth Michael Smith
• 金额: $ 17.01万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9333176
• 关键词: Anthrax Vaccine Absorbed; Anthrax Vaccines; Anthrax disease; Antibodies; Antibody Formation; Antibody Specificity; Antigens; B-Lymphocytes; Bacillus anthracis; Binding; Cells; Complex; Core Facility; Enzyme-Linked Immunosorbent Assay; Epitopes; Flow Cytometry; Foundations; Funding; Genus staphylococcus; Goals; Histones; Human; Immune response; Immunization; Immunoglobulin G; Immunoglobulin-Secreting Cells; Immunologics; Immunology; Immunotherapeutic agent; In Vitro; Individual; Infection; Inflammation; Influenza A Virus, H1N1 Subtype; Influenza B Virus; Length; Libraries; Medical Research; Memory; Memory B-Lymphocyte; Molecular; Molecular Conformation; Monoclonal Antibodies; Nucleosomes; Oklahoma; Organism; Pathogenicity; Patients; Peptidoglycan; Polysaccharides; Process; Production; Rabies virus; Reagent; Recording of previous events; Research; Research Personnel; Sepsis; Specificity; Streptococcus; Streptococcus pneumoniae; Systemic Lupus Erythematosus; Techniques; Technology; Testing; United Kingdom; Vaccinated; Vaccination; Vaccines; Vaccinia virus; Western Blotting; Work; anthrax lethal factor; anthrax protective factor; antibody libraries; base; depolymerization; experimental study; human monoclonal antibodies; in vivo; interest; member; new technology; novel; novel diagnostics; pandemic disease; pathogen; scale up; screening; tool; volunteer

The Human Antibody Core Facility is based on a technology that was developed at the Oklahoma Medical Research Foundation. The technology facilitates the production of fully human, full-length, antigen-specific monoclonal antibodies. The process can be completed in as little as 28 days post infection or immunization and creates full-length, properly paired antibodies of any desired IgG subclass. As a core facility, we have produced human monoclonals for a variety of purposes including novel diagnostics, passive immunotherapeutics and most importantly, understanding human immune responses to vaccination. We currently have a library of over nine hundred monoclonal antibodies. Known specificities include seasonal and pandemic H1N1, H3N2 and B influenza strains, S. pneumoniae capsular polysaccharide, rabies and vaccinia virus, and anthrax protective antigen (PA). In particular, our antibodies to anthrax protective antigen (PA), derived from an individual vaccinated with Anthrax Vaccine Absorbed (AVA), were carefully characterized, and two antibodies which bound to complex conformational epitopes of PA were shown to be protective against lethal toxin challenge both in vitro and in vivo. Our core facility produced these anti-PA monoclonals with previous funding as a member of the Cooperative Centers for Human Immunology during the last six years.

人类抗体核心设施是基于俄克拉荷马大学开发的一项技术