IGF::OT::IGF SBIR TOPIC 355: Cell and Animal-Based Models to Advance Cancer Health Disparity Research. Project Title: Establishment of 3D human tissue culture model system for advancing breast cancer

IGF::OT::IGF SBIR 主题 355：基于细胞和动物的模型推进癌症健康差异研究。

• 批准号: 9574791
• 负责人: Arun Bhardwaj
• 金额: $ 28.43万
• 依托单位: TATVA BIOSCIENCES, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-19 至 2018-06-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9574791
• 关键词: Advanced Malignant Neoplasm; African American; Animals; Applications Grants; Architecture; Area; Biological Models; Breast Cancer Patient; Cells; Characteristics; Development; Dimensions; Foundations; Generations; Genetic; Goals; Human Resources; Logistics; Mammary Neoplasms; Mass in breast; Modeling; Molecular; Outcome; Patients; Phase; Phenotype; Protocols documentation; Research; Small Business Innovation Research Grant; Standardization; Training; Validation; Woman; advanced system; base; cancer health disparity; caucasian American; human tissue; innovation; malignant breast neoplasm; molecular subtypes; novel; racial and ethnic; racial disparity; racial health disparity; three dimensional cell culture; three-dimensional modeling; tissue culture; tumor

Despite the recognition of obvious breast cancer (BC) racial-health disparity, we have failed to reduce the BC outcome gaps in African American women. Unavailability of three-dimensional (3D) tissue culture model systems that mimics the original tumor’s architecture and molecular characteristics of the racially disparate breast tumors limits our advancement in this area. The overall goal of this SBIR Phase-I proposal is to develop a standardized protocol for the establishment of a three-dimensional (3D) tissue culture model system using tumor mass from breast cancer patients of African American and Caucasian American racial/ethnic background. We will achieve overall goal in three parts: “A” development of 3D-tissue culture model system from racially disparate breast tumors; “B” phenotypic characterization of the developed 3D model system to confirm their stability and; “C” functional characterization and technical validation of the developed 3D-tissue culture models. Successful completion of this highly innovative proposal will result in a novel phenotypically stable 3D-culture model system that will be a landmark in BC racial disparity research. Moreover, this will provide a strong foundation for our next step, in that we will pursue a Phase II for developing up to 50 3D-tissue culture model system for racially disparate BC of different molecular subtypes.

尽管认识到明显的乳腺癌（BC）种族健康差异，但我们未能减少非裔美国妇女的BC结果差距。三维（3D）组织培养模型系统，模拟原始肿瘤的结构和不同种族的乳腺肿瘤的分子特征的不可用限制了我们在这一领域的进步。SBIR第一阶段提案的总体目标是开发一个标准化方案，用于使用来自非裔美国人和高加索美国人乳腺癌患者的肿瘤块建立三维（3D）组织培养模型系统 种族背景。我们将在三个部分实现总体目标：“A”从不同种族的乳腺肿瘤开发3D组织培养模型系统;“B”开发的3D模型系统的表型表征，以确认其稳定性;“C”开发的3D组织培养模型的功能表征和技术验证。成功完成这一高度创新的提案将产生一种新的表型稳定的3D文化模型系统，这将是BC种族差异研究的里程碑。此外，这将为我们的下一步提供坚实的基础，因为我们将进行第二阶段，为不同分子亚型的种族差异BC开发多达50个3D组织培养模型系统。