QUANTSTUDIO 12K FLEX OPEN ARRAY REAL-TIME PCR SYSTEM

项目摘要/摘要： 在这份S10提案中，我们申请了用于QUANTSTUDIO 12K Flex开放阵列实时PCR的资金 由Thermo/生命科学/ABI制造的系统，将使BMC和波士顿大学的用户能够 开展涉及基因表达分析、基因分型、microRNA图谱和其他RNA的研究 研究项目。这一创新和多功能的系统将取代旧的和过时的RT-PCR循环仪 在分析仪器核心(AIC)内，不再受制造商支持。 在过去的八年里，50多名首席调查人员及其实验室使用了AIC的 定量聚合酶链式反应仪器。这一新系统不仅将允许研究人员继续他们正在进行的研究，而且它 还将为新的和原创的项目创造机会。此外，拥有最新的软件分析 新文书的工具和更多能力将进一步促进多样性和有效性 核酸研究。它将允许用户使用不同的检测进行更广泛的分析 微米和纳米尺度的平台效率更高，并将加强数据处理。 Kenneth Walsh博士是该团队项目的首席主任和首席调查员(PD/PI)，他是 心血管疾病领域的顶尖科学家。他的实验室研究的分子事件 促进心肌细胞生长，从分化开始，以细胞死亡结束。科比博士会 继续使用AIC来确定自然遗传变异对 在药物滥用成瘾中赋予敏感度和韧性。迈尔斯博士将利用新的 QPCR系统通过进行miRNA图谱进一步研究基因和基因调控在两者中的作用 亨廷顿病(HD)和帕金森病(PD)。以上三位科学家构成了主要用户 组。 新仪器将使其他BU调查人员(主要和次要用户)能够检查 分子机制涉及多个研究领域，包括：代谢性心脏病(Dr。 Colucci)、阿尔茨海默病(AD)和肌萎缩侧索硬化症(ALS)(Wolozin博士和邱博士)， 糖尿病(Ruderman博士和Rameh-Plants博士)、肢体缺血血管形成(松井博士)、肥胖(Jing博士)、 钙信号(Bolotina博士)、乳腺癌和黑色素瘤(Thiagalingam博士和崔博士)、牙科疾病 (吉布森博士)、性传播疾病(韦茨勒博士)、病毒学(康纳博士)和肝病 (Bachschmidd博士)新仪器还将由AIC人员使用，以继续提供 为波士顿大学研究界的所有成员提供的“基因芯片服务”。 新仪器将被安置在AIC的qRT-PCR设施内，该设施位于AIC的2楼670号 奥尔巴尼街。AIC在维护仪器方面拥有丰富的经验，并确保 他们的工作条件很好，几乎没有停机时间。最后，AIC拥有长期的记录， 当涉及到RNA研究时，拥有出色的设备使用。

Project Summary/Abstract: In this S10 proposal, we request funds for a QUANTSTUDIO 12K FLEX OPEN ARRAY REAL-TIME PCR SYSTEM manufactured by Thermo/Life Science/ABI that will enable BMC and Boston University users to carry out studies involving gene expression analysis, genotyping, microRNA profiling, and other RNA research projects. This innovative and versatile system, will replace the old and obsolete RT-PCR cyclers within the Analytical Instrumentation Core (AIC) that are no longer being supported by the manufacturer. Over the past eight years, more than 50 principal investigators and their laboratories have used the AIC's qPCR instruments. This new system will not only allow researchers to continue their on-going studies, but it will also create opportunities for new and original projects. In addition, having the latest software analysis tools and the additional capabilities of the new instrument will further promote the diversity and efficacy of nucleic acid research. It will allow the users to perform a wider-range of assays using different detection platforms at both the micro and nano- scales much more efficiently and will enhance data processing. Dr. Kenneth Walsh is the Principal Director and Principal Investigator (PD/PI) for this team project and is a leading scientist in the field of cardiovascular disease. His laboratory examines the molecular events that promote cardiac cell muscle growth, starting from differentiation and ending with cell death. Dr. Bryant will continue to use the AIC to determine the contribution of natural genetic variation on the mechanisms that confer susceptibility vs. resilience in substance abuse addictions. Dr. Myers will take advantage of the new qPCR system by conducting miRNA profiling to further examine the role of genes and gene regulation in both Huntington's (HD) and Parkinson's Diseases (PD). The above three scientists composed the Major User Group. The new instrument will enable other BU investigators (both major and minor users) to examine the molecular mechanisms involved in various areas of research that include: metabolic heart disease (Dr. Colucci), Alzheimer's Disease (AD) and Amyotrophic Lateral Sclerosis (ALS) (Dr. Wolozin and Dr. Qiu), diabetes (Dr. Ruderman and Dr. Rameh-Plant), ischemic limb vascularization (Dr. Matsui), obesity (Dr. Jing), calcium signaling (Dr. Bolotina), breast cancer and melanoma (Dr. Thiagalingam and Dr. Cui), dental disease (Dr. Gibson), sexually transmitted diseases (STDs) (Dr. Wetzler), virology (Dr. Connor) and hepatic disease (Dr. Bachschmid). The new instrument will also be used by AIC personnel to continue being able to provide the “Gene Array Service” for all members of the BU research community. The new instrument will be housed within the AIC's qRT-PCR facility which is located on the 2nd floor at 670 Albany Street. The AIC has extensive experience when it comes to maintaining instruments and ensures that they are in excellent working condition with very little down time. Finally, the AIC has a long track record of having excellent equipment usage when it comes to RNA studies.