Oncology Tissue

肿瘤组织

• 批准号: 9519876
• 负责人: MARC K HALUSHKA
• 金额: $ 19.65万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9519876
• 关键词: Antibodies; Back; Biological Markers; Cancer Center Support Grant; Community Medicine; Comprehensive Cancer Center; Consultations; Custom; Cytogenetics; DNA; Databases; Funding; G-Banding; Growth; Histology; Image Analysis; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Individual; Laboratories; Malignant Neoplasms; Manuscripts; Metaphase; Molecular Analysis; Normal tissue morphology; Nucleic Acids; Online Systems; Organ; Procedures; Proteins; Protocols documentation; Publishing; RNA; Reporting; Research; Research Personnel; Research Support; Resolution; Resources; Scanning; Services; Slide; Software Tools; Speed; Stains; Technology; Tissue Harvesting; Tissue Microarray; Tissues; Translational Research; Tumor Tissue; Universities; base; clinical translation; design; digital; digital imaging; instrumentation; medical schools; member; new technology; novel; oncology; programs; quantitative imaging

ABSTRACT The Oncology Tissue Services (OTS) Core provides a number of technologies, services and scientific consultation to support the research of the Sidney Kimmel Comprehensive Cancer Center (SKCCC) members and other Johns Hopkins University (JHU) investigators. The Core was formerly known as the Tissue Microarray Core (TMA), when it provided TMA-related services, digital slide scanning and analysis, and routine histology services. With the addition of immunohistochemistry (IHC), immunofluorescence (IF) in situ hybridization (ISH) and the incorporation of the cytogenetics Core, the Core was renamed. It now provides traditional G-band metaphase analysis and FISH technologies, services added to this Core from a previous SKCCC CCSG Core. These expanded services enhance the ability of SKCCC researchers to perform tissue- based biomarker studies. The OTS Core has constructed greater than 826 TMAs for 55 investigators, containing tumor and normal tissue from most major organs. It has provided more than 80 new antibody workups and cored more than 2,500 blocks for RNA/DNA studies. It continues to provide essential cytogenetics services. Users published more than 104 manuscripts involving this resource since the last funding period began. In recent years, there has been tremendous growth in the service of providing tissue cores from FFPE tissue blocks for RNA and DNA molecular analyses. Instrumentation and expertise are available to automatically capture high-resolution digital images of both standard histology/IHC-based slides and TMA slides. The OTS Core obtained an automated slide stainer to convert laboratory discoveries of novel protein staining into clincal biomarkers. The Core provides Web-based software tools and two back-end databases (TMAJ/Spectrum) to facilitate all facets of research involving TMAs, routine slides and quantitative image analysis. The specific aims of this Core are: Aim 1: Provide affordable, high-quality histology, immunohistochemistry, cytogenetics, nucleic acid harvesting from tissues, digital slide scanning and analysis, and tissue microarray construction services. Aim 2: Identify and develop new technologies to support and speed translational research in cancer, including automated in situ hybridization and clinical translation of research immunohistochemistry discoveries. Aim 3: Support the Research Programs and respond to the needs of its users to by working with specific investigators on new protocols, procedures and custom procedures to meet their evolving histology, immunohistochemistry and slide scanning requirements. SKCCC Managed Core Reporting Period: Jan. 1, 2015, to Dec. 31, 2015

摘要 肿瘤组织服务（OTS）核心提供了许多技术，服务和科学 悉尼金梅尔综合癌症中心（SKCCC）成员的研究咨询 和其他约翰霍普金斯大学（JHU）的研究人员。核心以前被称为组织 微阵列核心（TMA），当它提供TMA相关的服务，数字幻灯片扫描和分析，以及常规 组织学服务。随着免疫组化（IHC）、原位免疫荧光（IF） 通过杂交（ISH）和掺入细胞遗传学核心，将核心重新命名。它现在提供 传统的G带中期分析和FISH技术，从以前的服务添加到这个核心 SKCCC CCSG核心。这些扩展的服务提高了SKCCC研究人员进行组织- 生物标志物研究。OTS核心已经为55名研究者构建了超过826个TMA， 包含肿瘤和大多数主要器官的正常组织。它提供了80多种新的抗体 检查和取芯超过2,500块用于RNA/DNA研究。它继续提供必要的 细胞遗传学服务。自去年以来，用户发表了超过104篇涉及此资源的手稿 融资期开始了。近年来，提供组织的服务有了巨大的增长 用于RNA和DNA分子分析的FFPE组织块核心。仪器和专业知识 可自动捕获标准组织学/IHC切片的高分辨率数字图像 和TMA幻灯片OTS核心获得了一台自动化载玻片染色机，将实验室发现的新的 蛋白质染色转化为临床生物标志物。核心提供基于Web的软件工具和两个后端 数据库（TMAJ/Spectrum），以促进研究的各个方面，包括TMA、常规幻灯片和定量分析。 图像分析该核心的具体目标是： 目标1：提供经济实惠、高质量的组织学、免疫组织化学、细胞遗传学、核酸收获 从组织，数字切片扫描和分析，以及组织微阵列构建服务。 目标2：识别和开发新技术，以支持和加速癌症转化研究，包括 自动原位杂交和临床翻译研究免疫组织化学发现。 目标3：支持研究项目，并通过与特定的研究项目合作， 研究人员对新的协议，程序和定制程序，以满足他们不断发展的组织学， 免疫组织化学和载玻片扫描要求。 SKCCC托管核心 报告期：2015年1月1日至2015年12月31日