Sequence-specific Hybridization Capture for Discovery of Proteoform–lncRNA Interactions in Prostate Cancer
用于发现前列腺癌中 Proteoform-lncRNA 相互作用的序列特异性杂交捕获
基本信息
- 批准号:9887230
- 负责人:
- 金额:$ 38.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-03-04 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:AftercareAggressive courseApoptosisBiologicalBiological AssayBiological MarkersBlood capillariesCellsCessation of lifeChromatinClinicalComplexDatabasesDevelopmentDiagnosisDiseaseDisease ManagementDisease ProgressionEconomicsEvaluationGene ExpressionGenetic TranscriptionGenomicsGrantGrowthHIVHumanImmunoprecipitationIndividualIndolentInformaticsIntelligenceMALAT1 geneMYC geneMalignant NeoplasmsMalignant neoplasm of prostateMass Spectrum AnalysisMessenger RNAMonitorNatureNeoplasm MetastasisOutcomePatientsPeptidesPhasePlayProcessPrognostic MarkerProstate-Specific AntigenProstatic NeoplasmsProteinsProteomicsQuality of lifeRNARNA ProcessingRNA SplicingRNA purificationRNA-Binding ProteinsRegulationResearch PersonnelResistanceRoleSamplingSignal TransductionSiteSoftware ToolsSolid NeoplasmSystemTechniquesTechnologyTestingTimeTissue SampleTranscriptTranslationsUnited StatesUntranslated RNAVariantWorkXenograft procedureangiogenesisc-myc Genescancer cellcancer diagnosisdesigndifferential expressiongenomic RNAhuman tissueimprovedinsightinstrumentmenminimally invasivemultiple data typesnew technologynew therapeutic targetnovelprognostic assaysprogramsprotein complexproteomic signaturerecruitscaffoldsuccesstooltranscriptome sequencingtumor
项目摘要
Project Summary/Abstract
Long noncoding RNAs (lncRNAs) are vital components of gene expression programs controlling cellular
differentiation and function. lncRNAs act as scaffolds to recruit or sequester effector proteins. They may act in
cis and trans at multiple genomic sites. lncRNAs modulate transcription, chromatin organization, RNA
processing, and translation, and many questions remain unanswered regarding how they influence gene
expression. Recent reviews have detailed several lncRNAs that play key roles in prostate cancer including
regulation of processes in cancer cells such as proliferative signaling, replicative immortality, invasion and
metastasis, evasion of growth suppressors, induction of angiogenesis and resistance to apoptosis. While much
remains to be learned regarding the mechanisms of action involved in lncRNA function, it is abundantly clear
that lncRNAs act in concert with associated proteins to carry out their roles.
Distinguishing between aggressive and indolent prostate cancer is a major conundrum in cancer. In roughly
one-third of the 140,000 intermediate grade cancers (Gleason 7) diagnosed annually, the disease follows a
more aggressive course developing rapid progression of prostate specific antigen (PSA) after treatment and
earlier metastasis and death. During the prior three-year grant period, we discovered several dozen lncRNAs
that distinguish aggressive and indolent prostate cancers, of which four were validated using RT-qPCR. We
also developed the HyPR-MS strategy to permit elucidation of specific RNA-protein interactomes.
In this renewal proposal we seek to further develop and apply a suite of powerful new proteomics tools to study
these and other prostate cancer-relevant lncRNAs and the proteins that associate with them, and use this
capability to reveal important proteomic signatures to distinguish aggressive versus indolent prostate cancer.
These studies will provide a novel and unprecedented view into the nature of the proteoform–lncRNA
interactions that underlie lncRNA function. The delineation of differentially expressed lncRNAs and their
associated proteins/proteoforms will provide insight into the biological mechanisms underlying disease
progression and provide novel therapeutic targets for further development.
项目总结/文摘
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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{{ truncateString('DAVID F. JARRARD', 18)}}的其他基金
Sequence-specific Hybridization Capture for Discovery of Proteoform–lncRNA Interactions in Prostate Cancer
用于发现前列腺癌中 Proteoform-lncRNA 相互作用的序列特异性杂交捕获
- 批准号:
10541119 - 财政年份:2015
- 资助金额:
$ 38.88万 - 项目类别:
Sequence-specific Capture for Discovering Protein-IncRNA Interactions in Prostate Cancer
用于发现前列腺癌中蛋白质-IncRNA 相互作用的序列特异性捕获
- 批准号:
8857740 - 财政年份:2015
- 资助金额:
$ 38.88万 - 项目类别:
Sequence-specific Hybridization Capture for Discovery of Proteoform–lncRNA Interactions in Prostate Cancer
用于发现前列腺癌中 Proteoform-lncRNA 相互作用的序列特异性杂交捕获
- 批准号:
10307993 - 财政年份:2015
- 资助金额:
$ 38.88万 - 项目类别:
Modulation Of IGF-II Imprinting in the Aging Prostate
老化前列腺中 IGF-II 印记的调节
- 批准号:
7656965 - 财政年份:2008
- 资助金额:
$ 38.88万 - 项目类别:
Mechanisms for Acquired Changes in Prostate Growth Regulation
前列腺生长调节的获得性变化的机制
- 批准号:
7500390 - 财政年份:2007
- 资助金额:
$ 38.88万 - 项目类别:
MODULATION OF IGF-II IMPRINTING IN THE AGING PROSTATE
老化前列腺中 IGF-II 印记的调节
- 批准号:
6769427 - 财政年份:2002
- 资助金额:
$ 38.88万 - 项目类别:
MODULATION OF IGF-II IMPRINTING IN THE AGING PROSTATE
老化前列腺中 IGF-II 印记的调节
- 批准号:
7077643 - 财政年份:2002
- 资助金额:
$ 38.88万 - 项目类别:
Modulation of IGF-II Imprinting in the Aging Prostate
老化前列腺中 IGF-II 印记的调节
- 批准号:
7793492 - 财政年份:2002
- 资助金额:
$ 38.88万 - 项目类别: