Sequence-specific Hybridization Capture for Discovery of Proteoform–lncRNA Interactions in Prostate Cancer

用于发现前列腺癌中 Proteoform-lncRNA 相互作用的序列特异性杂交捕获

基本信息

  • 批准号:
    9887230
  • 负责人:
  • 金额:
    $ 38.88万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-03-04 至 2024-12-31
  • 项目状态:
    已结题

项目摘要

Project Summary/Abstract Long noncoding RNAs (lncRNAs) are vital components of gene expression programs controlling cellular differentiation and function. lncRNAs act as scaffolds to recruit or sequester effector proteins. They may act in cis and trans at multiple genomic sites. lncRNAs modulate transcription, chromatin organization, RNA processing, and translation, and many questions remain unanswered regarding how they influence gene expression. Recent reviews have detailed several lncRNAs that play key roles in prostate cancer including regulation of processes in cancer cells such as proliferative signaling, replicative immortality, invasion and metastasis, evasion of growth suppressors, induction of angiogenesis and resistance to apoptosis. While much remains to be learned regarding the mechanisms of action involved in lncRNA function, it is abundantly clear that lncRNAs act in concert with associated proteins to carry out their roles. Distinguishing between aggressive and indolent prostate cancer is a major conundrum in cancer. In roughly one-third of the 140,000 intermediate grade cancers (Gleason 7) diagnosed annually, the disease follows a more aggressive course developing rapid progression of prostate specific antigen (PSA) after treatment and earlier metastasis and death. During the prior three-year grant period, we discovered several dozen lncRNAs that distinguish aggressive and indolent prostate cancers, of which four were validated using RT-qPCR. We also developed the HyPR-MS strategy to permit elucidation of specific RNA-protein interactomes. In this renewal proposal we seek to further develop and apply a suite of powerful new proteomics tools to study these and other prostate cancer-relevant lncRNAs and the proteins that associate with them, and use this capability to reveal important proteomic signatures to distinguish aggressive versus indolent prostate cancer. These studies will provide a novel and unprecedented view into the nature of the proteoform–lncRNA interactions that underlie lncRNA function. The delineation of differentially expressed lncRNAs and their associated proteins/proteoforms will provide insight into the biological mechanisms underlying disease progression and provide novel therapeutic targets for further development.
项目总结/文摘

项目成果

期刊论文数量(0)
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专利数量(0)

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DAVID F. JARRARD其他文献

DAVID F. JARRARD的其他文献

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{{ truncateString('DAVID F. JARRARD', 18)}}的其他基金

University of Wisconsin Prostate SPORE
威斯康星大学前列腺孢子
  • 批准号:
    10555398
  • 财政年份:
    2023
  • 资助金额:
    $ 38.88万
  • 项目类别:
Administrative Core
行政核心
  • 批准号:
    10555399
  • 财政年份:
    2023
  • 资助金额:
    $ 38.88万
  • 项目类别:
Sequence-specific Hybridization Capture for Discovery of Proteoform–lncRNA Interactions in Prostate Cancer
用于发现前列腺癌中 Proteoform-lncRNA 相互作用的序列特异性杂交捕获
  • 批准号:
    10541119
  • 财政年份:
    2015
  • 资助金额:
    $ 38.88万
  • 项目类别:
Sequence-specific Capture for Discovering Protein-IncRNA Interactions in Prostate Cancer
用于发现前列腺癌中蛋白质-IncRNA 相互作用的序列特异性捕获
  • 批准号:
    8857740
  • 财政年份:
    2015
  • 资助金额:
    $ 38.88万
  • 项目类别:
Sequence-specific Hybridization Capture for Discovery of Proteoform–lncRNA Interactions in Prostate Cancer
用于发现前列腺癌中 Proteoform-lncRNA 相互作用的序列特异性杂交捕获
  • 批准号:
    10307993
  • 财政年份:
    2015
  • 资助金额:
    $ 38.88万
  • 项目类别:
Modulation Of IGF-II Imprinting in the Aging Prostate
老化前列腺中 IGF-II 印记的调节
  • 批准号:
    7656965
  • 财政年份:
    2008
  • 资助金额:
    $ 38.88万
  • 项目类别:
Mechanisms for Acquired Changes in Prostate Growth Regulation
前列腺生长调节的获得性变化的机制
  • 批准号:
    7500390
  • 财政年份:
    2007
  • 资助金额:
    $ 38.88万
  • 项目类别:
MODULATION OF IGF-II IMPRINTING IN THE AGING PROSTATE
老化前列腺中 IGF-II 印记的调节
  • 批准号:
    6769427
  • 财政年份:
    2002
  • 资助金额:
    $ 38.88万
  • 项目类别:
MODULATION OF IGF-II IMPRINTING IN THE AGING PROSTATE
老化前列腺中 IGF-II 印记的调节
  • 批准号:
    7077643
  • 财政年份:
    2002
  • 资助金额:
    $ 38.88万
  • 项目类别:
Modulation of IGF-II Imprinting in the Aging Prostate
老化前列腺中 IGF-II 印记的调节
  • 批准号:
    7793492
  • 财政年份:
    2002
  • 资助金额:
    $ 38.88万
  • 项目类别:
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