Experimental Pathology Laboratory Shared Resource

项目摘要/摘要 实验病理学实验室(EPL)的使命是提供一系列全面的 组织病理学服务和专业知识，使UWCCC成员能够充分利用动物的力量 人类癌症模型，揭示癌症发生、发展和发展的机制 对治疗的反应性。EPL的工作人员在组织学、组织病理学和癌症方面拥有广泛的专业知识 生物学和Together在癌症研究方面拥有近100年的经验。EPL总共服务了99名 UWCCC成员在当前CCSG供资周期内，每年43至58个独立成员 在此时间间隔内使用的EPL服务。使用EPL的会员数量和服务量 自上次审查CCSG以来，提供的数据有所增加，2016年对成员的调查数据表明 使用量将继续增加。EPL优先考虑以下三个目标：目标1是提供基本的组织学 为UWCCC成员提供的服务。我们的成员利用各种各样的动物模型来处理重要的 关于癌症病因学、预防和治疗的问题。EPL通过提供以下信息支持这项研究 基本的组织学服务和专业知识。UWCCC调查人员收集的组织样本包括 传输到EPL，在那里根据 调查人员的要求和我们标准化的操作程序。EPL提供常见的染色 例如苏木精和伊红(H&E)，几乎可以进行任何特殊染色。专家 病理咨询由Ruth Sullivan博士提供，Ruth Sullivan博士是委员会认证的兽医病理学家和EPL工作人员 成员。目的2是为了促进正常组织和肿瘤组织的分子分析。UWCCC成员 经常评估作为生物标志物或治疗性的特定蛋白质的表达、功能或定位 目标。应成员要求，EPL将开发和实施定制免疫组织化学 (IHC)或免疫荧光(IF)针对感兴趣的蛋白质。我们的成员认为这一点 服务是无价的，有70多个独特的IHC，或者协议已经开发、优化和验证 在当前CCSG供资周期内由EPL提供。目标3是为UWCCC提供组织病理学专业知识 成员和研究人员。EPL工作人员可即场咨询样品制备 以及操作或操作我们的尼康荧光显微镜进行图像采集。沙利文博士提供了 每月免费向UWCCC研究人员提供幻灯片评论和教程。此外，沙利文博士有空 做普通的病理学咨询。CCSG的支持使我们能够为UWCCC成员提供优先 以低于商业实验室的成本获得高质量的组织病理学服务，以及 向成员免费提供专家咨询支持。

PROJECT SUMMARY / ABSTRACT The mission of the Experimental Pathology Laboratory (EPL) is to provide a comprehensive array of histopathology services and expertise that enable UWCCC members to fully exploit the power of animal models of human cancer to reveal mechanisms that contribute to cancer development, progression, and responsiveness to therapies. EPL staff have extensive expertise in histology, histopathology, and cancer biology and together possess nearly 100 years of experience in cancer research. EPL has served a total of 99 UWCCC members during the current CCSG funding cycle, and between 43 and 58 unique members per year used EPL services during this interval. Both the number of members using EPL and the volume of services provided have increased since the CCSG was last reviewed and data from a 2016 survey of members indicate use will continue to increase. EPL prioritizes the following three aims: Aim 1 is to provide essential histology services to UWCCC members. Our members utilize a wide variety of animal models to address important questions pertaining to cancer etiology, prevention, and therapy. The EPL supports this research by providing essential histology services and expertise. Tissue specimens collected by UWCCC investigators are transported to the EPL where they are processed, embedded, sectioned, and stained according to the requirements of the investigator and our Standardized Operating Procedures. EPL offers common staining protocols, for example hematoxylin and eosin (H&E), and can perform virtually any special stain. Expert pathology consultation is provided by Dr. Ruth Sullivan, a board certified veterinary pathologist and EPL staff member. Aim 2 is to facilitate the molecular analyses of normal and neoplastic tissues. UWCCC members frequently evaluate expression, function, or localization of specific proteins as biomarkers or therapeutic targets. Upon request of a member, the EPL will develop and implement a custom immunohistochemistry (IHC) or immunofluorescence (IF) protocol that is specific to a protein of interest. Our members view this service as invaluable and over 70 unique IHC or IF protocols have been developed, optimized, and validated by EPL during the current CCSG funding cycle. Aim 3 is to provide histopathology expertise to UWCCC members and researchers. EPL staff members are available for walk-in consultation on sample preparation and handling or on operation of our Nikon fluorescence microscope for image acquisition. Dr. Sullivan provides monthly slide reviews and tutorials to UWCCC researchers free of charge. In addition, Dr. Sullivan is available for general pathology consults. Support from the CCSG allows us to provide UWCCC members with priority access to high quality histopathology services at costs below those of commercial laboratories as well as to provide expert consultative support at no cost to members.