Mechanisms of mRNA regulation by La-related protein 1

La相关蛋白1对mRNA的调控机制

• 批准号: 9894154
• 负责人: Andrea J. Berman
• 金额: $ 3.4万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9894154
• 关键词: 8 year old; Affinity; Biological Process; Biological Sciences; Collection; Computer software; Computers; Contracts; Crystallization; Data; Data Analyses; Funding; Gel; Grant; Image; Laboratories; Licensing; Link; Maintenance; Messenger RNA; National Institute of General Medical Sciences; Proteins; RNA Recognition Motif; RNA-Binding Proteins; Radioisotopes; Regulation; Resolution; Running; Scanning; Time; Transcript; Translations; Universities; base; cost; experimental study; imager; instrument

Summary/Abstract Funds from NIGMS are requested for the purchase of an Amersham Typhoon IP phosphorimager, associated storage phosphor imaging plates and cassettes, and software licenses for data analysis. The institutional commitments from the University of Pittsburgh and its Department of Biological Sciences toward this endeavor include trading in the old imager ($5,000), purchasing the computer to run the instrument (~$2,500), contributing $6,000 to defray the cost of the request, and providing the maintenance contract. The imager scans storage phosphor screens up to 34 x 46 cm quickly and to high resolution, facilitating the collection of quantitative data over a large dynamic range. The radionuclide-based experiments in the Berman and Brodsky laboratories in the Department of Biological Sciences are currently imaged using an >8-year old GE Typhoon FLA 7000 phosphorimager, whose resolution and dynamic range are significantly lower than those of the request instrument. This imager uses glitchy, but no longer supported, software. Both the hardware and software routinely malfunction, resulting in days of wasted time; it also only accommodates exposed gels that are up to 20 x 40 cm. The request of funding to purchase the Amersham Typhoon IP phosphorimager is linked to grant R01 GM116889 to Dr. Andrea Berman and grant R01 GM075061 to Prof. Jeffrey Brodsky. Each aim described in grant R01 GM116889 to Dr. Berman necessitates the use of trace levels of radioisotopes, which allow for quantitative assessment of high-affinity interactions. These analyses are critical first-steps in identifying appropriate co-crystallization targets and in characterizing biological function. Thus, a reliable, functioning phosphorimager with high sensitivity is essential to the successful completion of the aims.

总结/摘要 请荷兰地理信息系统提供资金，用于购买一台阿默舍姆台风IP磷光成像仪， 存储荧光成像板和暗盒，以及用于数据分析的软件许可证。体制 匹兹堡大学及其生物科学系对这一奋进的承诺 包括旧成像仪的交易（5,000美元），购买运行仪器的计算机（约2,500美元）， 捐助6 000美元以支付请求的费用，并提供维修合同。成像器 扫描存储荧光屏高达34 x 46厘米快速和高分辨率，便于收集 大动态范围内的定量数据。Berman和Brodsky的放射性核素实验 生物科学系的实验室目前使用一台使用超过8年的GE台风成像仪进行成像 弗拉7000磷光成像仪，其分辨率和动态范围明显低于 请求文书。这款相机使用的软件不稳定，但不再受支持。硬件和 软件经常出现故障，导致浪费数天的时间;它也只容纳暴露的凝胶， 最大为20 x 40 cm。购买阿默舍姆台风IP磷光成像仪的资金请求与 授予Andrea Berman博士R 01 GM 116889，授予Jeffrey Brodsky教授R 01 GM 075061。每个目标 在授予Berman博士的授权R 01 GM 116889中描述的放射性同位素的使用需要使用痕量水平的放射性同位素， 允许高亲和力相互作用的定量评估。这些分析是关键的第一步， 鉴定合适的共结晶靶点和表征生物学功能。一个可靠的， 具有高灵敏度的磷光成像仪对于成功实现这些目标至关重要。