Mechanisms of mRNA regulation by La-related protein 1

摘要/摘要 请从NIGMS获得资金，用于购买一台amersham Typhoon IP磷成像仪，相关 存储荧光粉成像板和盒式磁带，以及用于数据分析的软件许可证。体制上的 匹兹堡大学及其生物科学系对这一努力的承诺 包括旧成像仪的交易(5000美元)，购买运行仪器的计算机(约2500美元)， 捐助6 000美元以支付请求费用，并提供维修合同。成像器 快速扫描高达34 x 46厘米的储存式荧光屏，分辨率高，便于收集 大动态范围内的定量数据。伯曼和布罗茨基的放射性核素实验 生物科学部的实验室目前正在使用8岁的GE台风进行成像 FLA 7000磷像仪，其分辨率和动态范围明显低于 请求仪器。这款相机使用的是有问题但不再受支持的软件。硬件和 软件经常出现故障，导致数天的时间浪费；它也只容纳暴露的凝胶 高达20 x 40厘米。为购买amersham Typhoon IP磷像仪提供资金的请求与 授予Andrea Berman博士R01 GM116889，授予Jeffrey Brodsky教授R01 GM075061。每个目标 在授予Berman博士的R01 GM116889授权中描述的需要使用痕量放射性同位素，这 允许对高亲和力相互作用进行定量评估。这些分析是关键的第一步 确定适当的共结晶目标和表征生物功能。因此，一个可靠的， 正常工作的高灵敏度磷成像仪是成功完成目标的关键。

Summary/Abstract Funds from NIGMS are requested for the purchase of an Amersham Typhoon IP phosphorimager, associated storage phosphor imaging plates and cassettes, and software licenses for data analysis. The institutional commitments from the University of Pittsburgh and its Department of Biological Sciences toward this endeavor include trading in the old imager ($5,000), purchasing the computer to run the instrument (~$2,500), contributing $6,000 to defray the cost of the request, and providing the maintenance contract. The imager scans storage phosphor screens up to 34 x 46 cm quickly and to high resolution, facilitating the collection of quantitative data over a large dynamic range. The radionuclide-based experiments in the Berman and Brodsky laboratories in the Department of Biological Sciences are currently imaged using an >8-year old GE Typhoon FLA 7000 phosphorimager, whose resolution and dynamic range are significantly lower than those of the request instrument. This imager uses glitchy, but no longer supported, software. Both the hardware and software routinely malfunction, resulting in days of wasted time; it also only accommodates exposed gels that are up to 20 x 40 cm. The request of funding to purchase the Amersham Typhoon IP phosphorimager is linked to grant R01 GM116889 to Dr. Andrea Berman and grant R01 GM075061 to Prof. Jeffrey Brodsky. Each aim described in grant R01 GM116889 to Dr. Berman necessitates the use of trace levels of radioisotopes, which allow for quantitative assessment of high-affinity interactions. These analyses are critical first-steps in identifying appropriate co-crystallization targets and in characterizing biological function. Thus, a reliable, functioning phosphorimager with high sensitivity is essential to the successful completion of the aims.