Waters ACQUITY UPLC M-Class with NanoLockSpray ionization source

配备 NanoLockSpray 电离源的沃特世 ACQUITY UPLC M-Class

• 批准号: 9893408
• 负责人: Kelly Keisen Lee
• 金额: $ 8.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9893408
• 关键词: Biological Process; Bos taurus structural-GP protein; Cells; Environment; Enzymes; Equipment; Funding; Grant; High Pressure Liquid Chromatography; Liquid Chromatography; Mass Spectrum Analysis; Membrane; Membrane Glycoproteins; Membrane Proteins; National Institute of General Medical Sciences; Pharmaceutical Preparations; Pharmacy Schools; Proteins; Sampling; Source; Structural Protein; System; Techniques; Therapeutic Intervention; Viral; Virus; Water; drug metabolism; glycoprotein structure; interest; ionization; nanoscale; protein structure; targeted treatment; tool

ABSTRACT The current equipment supplement request is for an upgrade of the current mass spectrometry platform to enable nano-scale liquid chromatography. Active grants R01 GM099989 (PI: Lee), and R01s GM130810 and GM121603 (PI: Atkins) jointly request the funds for a Waters M-class HPLC with the corresponding NanoLockSpray ionization source. The upgrade will provide the enhancement in sensitivity to overcome the limits in sensitivity and material requirements that have hindered analytical characterization of numerous the proteins of interest. This will dramatically increase the range of systems and samples we can analyze. The equipment will be shared equally among three NIGMS-funded PIs while being managed and maintained by the School of Pharmacy Mass Spectrometry Facility.

摘要 目前的设备补充要求是对目前的质谱仪进行升级 该平台可实现纳米级液相色谱。活动赠款R01 GM099989（PI：Lee）， 和R 01的GM 130810和GM 121603（PI：阿特金斯）共同申请沃茨M级的资金 使用相应的NanoLockSpray电离源进行HPLC。升级将提供 提高灵敏度，以克服灵敏度和材料要求的限制， 阻碍了许多感兴趣的蛋白质的分析表征。这将 大大增加了我们可以分析的系统和样品的范围。这些设备将 由三个NIGMS资助的项目执行机构平均分摊， 药学院质谱设备。