Physiology Core

摘要 生理学核心的主要目标是为刚果民主共和国成员提供访问中央设施的途径， 解决与糖尿病相关的复杂新陈代谢问题的服务和技术专长， 糖尿病或转基因啮齿动物模型。核心分为两个子核心，即动物外科 和实验程序子核和分析子核，每个子核都包含专门的 帮助刚果(金)调查人员和/或其受训人员最大限度地完成任务的设备和关键人员 高效率和高成本效益的方式。它也是不同成员之间合作的论坛 研究背景，但对研究糖尿病有共同的兴趣。通过动物外科和 实验程序分核心，刚果民主共和国调查人员获得培训、设备和实验室设施 和技术专长，以执行复杂的啮齿动物手术，包括放置血管导管和 其他植入物，如脑微注射和微透析探头。核心员工帮助实施复杂的 使用专门的实验方法(如葡萄糖钳、示踪剂、微透析)进行代谢研究 和电流测量研究)--研究人员不容易掌握的技术 没有以前的培训或经验，特别是年轻的调查人员、研究员和飞行员获奖者。 分析分核心为刚果民主共和国成员提供了一个用于测量 专门从啮齿动物研究中提取的糖调节激素、细胞因子和神经递质。这 生理学核心的组成部分受益于持续和多产的 放射免疫分析和高效液相色谱分析设备。扩展了基于质谱学的分析方法和方法 细胞因子和神经递质DRC-MS共享资源的建立 并提供最先进的蛋白质检测和翻译后修饰的蛋白质组学方法 量化将有助于极大地提高这一核心对刚果民主共和国调查员社区的价值。一起， 这两个子核心为刚果民主共和国成员提供了系统地处理相关问题的独特机会 在活体中的机制问题并评估中枢神经系统和 以最有效和最经济的方式保护周围组织。

Abstract The primary objective of the Physiology Core is to provide DRC members with access to centralized facilities, services and technical expertise to address complex metabolic questions related to diabetes using normal, diabetic or genetically modified rodent models. The core is structured into two Sub-cores, the Animal Surgery and Experimental Procedure Sub-core and the Analytical Sub-core, each of which contains specialized equipment and key personnel to help DRC investigators and/or their trainees achieve their tasks in the most efficient and cost-effective manner. It also serves as a forum for collaboration between members with different research backgrounds, but a common interest in studying diabetes. Through the Animal Surgery and Experimental Procedure Sub-core, DRC investigators gain access to training, equipment, laboratory facilities and technical expertise to perform complex rodent surgeries, including the placement of vascular catheters and other implantables, such as brain micro-injection and micro-dialysis probes. Core staff help carry out complex metabolic studies using specialized experimental methodologies (e.g. glucose clamps, tracers, microdialysis and amperometric studies) in conscious mice and rats – skills that are not easily accessible to investigators without previous training or experience, particularly young investigators, fellows, and pilot award recipients. The Analytical Sub-core provides DRC members with a central facility for the measurement of glucoregulatory hormones, cytokines and neurotransmitters specifically derived from rodent studies. This component of the Physiology Core benefits from the expertise and equipment of an on-going and prolific radioimmunoassay and HPLC facility. Expansion of the repertoire of mass spectrometry-based assays and establishment of a DRC - Mass Spectrometry Shared Resource to measure cytokines and neurotransmitters and to offer state-of-the-art proteomic methods of protein detection and post-translational modification quantification will serve to greatly enhance the value of this core to the DRC investigator community. Together, these two sub-cores provide DRC members with the unique opportunity to systematically address pertinent mechanistic questions in vivo and to assess metabolic changes in both the central nervous system and peripheral tissues in the most efficient and economical manner.