ShEEP Equipment Request for GeoMx™ Digital Spatial Profiler System

本申请是为了获得GeoMx™数字空间剖面仪系统（NanoString技术），代表 旧金山弗朗西斯科医学中心（SFVAMC）Skeleton生物学和生物力学核心设施， 最初成立于2003年，由BLR&D研究增强奖计划资助，后来成为 BLR&D计划项目这个核心已经支持了70多个VA，NIH和国防部资助的项目， 内分泌学、矫形外科、神经生物学、免疫学和心胸外科， 在更大的UCSF研究范围内的基础和转化研究的许多新的合作 社区拟议收购的这一最先进的GeoMx™数字空间剖面仪系统， 优化，以促进生物标本和结果的分析，旨在进一步扩大我们的核心 翻译和临床研究能力。该工具将允许调查人员访问该核心， 研究动物研究中细胞和组织的细胞，分子和生物化学特征， 以高通量和高定量的方式对患者进行活检。考虑到分子的关键作用 变化（RNA和蛋白质表达）在整个发展和疾病的生物过程中，这 设备将使我们的研究人员能够促进现有的研究和追求未探索的研究领域 其效率和准确性是目前核心中的仪器无法实现的。这 新的仪器预计在短期内大大扩大研究范围的9个积极的VA- 资助的项目和超过9个NIH和DoD项目授予参与研究者。

This application is to acquire a GeoMx™ Digital Spatial Profiler System (NanoString Technology) on behalf of the San Francisco VA Medical Center (SFVAMC) Skeletal Biology and Biomechanics Core Facility, which was originally established in 2003 by funding of BLR&D Research Enhancement Award Program and later a BLR&D Program Project. This Core had supported more than 70 VA, NIH, and DoD-funded projects in endocrinology, orthopedics surgery, neurobiology, immunology, and cardiothoracic surgery, and enabled numerous new collaborations in basic and translational research within the greater UCSF research community. The proposed acquisition of this state-of-the-art GeoMx™ Digital Spatial Profiler System, which is optimized to facilitate analyses of biological specimens and outcomes, is aimed to further expand our Core capacity for translational and clinical investigation. This tool will allow investigators accessing this Core to investigate the cellular, molecular, and biochemical features of cells and tissues from animal studies and patient biopsies in a high-throughput and highly quantitative manner. Given the critical role of the molecular changes (RNA and protein expression) in biological processes throughout development and disease, this equipment would allow our investigators to facilitate existing studies and pursue unexplored area of research with an efficiency and accuracy that could not be achieved by the current instrumentation in the Core. This new instrumentation is anticipated in the short term to greatly expand the research scope of 9 active VA- funded projects and more than 9 NIH and DoD projects awarded to the participating investigators.