Cytometry and Microscopy Shared Resource

细胞计数和显微镜共享资源

• 批准号: 9904139
• 负责人: Brian T. Grimberg
• 金额: $ 21.52万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9904139
• 关键词: Accounting; Acoustics; Address; Applications Grants; Appointment; Area; Automation; Biological Assay; Cancer Center Support Grant; Cell Cycle; Cell Death; Cell Separation; Cells; Clinic; Clinical; Clinical Investigator; Clinical Trials; Communication; Comprehensive Cancer Center; Consult; Consultations; Cost Savings; Cytometry; Development; Distant; Environmental Health; Equipment; Financial Support; Funding; Future; Glioma; Goals; Grant; Guidelines; Hypoxia; Immunologic Monitoring; Immunophenotyping; Lasers; Malignant Neoplasms; Manufacturer Name; Measurement; Microscope; Microscopy; Mission; Nature; Paper; Pathway Analysis; Pathway interactions; Pharmaceutical Preparations; Play; Ploidies; Proteins; Publishing; Radiation; Research; Research Assistant; Research Institute; Research Personnel; Research Support; Resource Sharing; Role; Safety; Science; Services; Shapes; Site; Supporting Cell; Training; United States National Institutes of Health; Universities; University Hospitals; Validation; aged; anticancer research; assay development; base; cancer therapy; cellular imaging; data sharing networks; design; drug discovery; imaging facilities; instrument; medical schools; meetings; member; programs; response

The Cytometry and Microscopy Shared Resource (Cytometry SR) is an essential asset to Case Comprehensive Cancer Center (Case CCC) members' research, illustrated by the breadth of scientific program involvement and reflected in the large number of investigators who use this Shared Resource (SR). Last year alone, over 300 investigators, 37% of whom were Case CCC members, accounting for 40% of total usage, from all 7 of the Case CCC Programs used the SR. The mission of the Cytometry SR is to support cell-based assays in basic, translational, and clinical cancer research and clinical trials. This multi-site SR, jointly supported and managed by the Case Comprehensive Cancer Center (Case CCC), Case Western Reserve University (CWRU) School of Medicine (SOM), University Hospitals (UH), and Cleveland Clinic (CC) Lerner Research Institute (LRI), provides formal access to expensive instruments, and professional expertise and assistance in the areas of cytometry, microscopy, and cell sorting. The Specific Aims of the Cytometry SR are to: 1. Provide well-maintained flow cytometers, cell sorters, microscopes, and associated equipment. 2. Provide training and consultation by cytometry and microscopy professional staff. 3. Provide full or partial cell-based assay development to support clinical investigators. At last review, the Cytometry SR was rated Excellent to Very Good Merit. Throughout the current funding cycle (2012-2016), the Cytometry SR provided services to 484 registered users, 132 (27%) of whom are Case CCC members, representing all 7 of the Case CCC programs. The Cytometry SR provides current, well-maintained instruments that perform within the manufacturer’s specifications and are compliant with the safety guidelines from Environmental Health and Safety (EHS), Departments (CWRU, CC), and NIH. The SR Director, Co-Directors, Manager, and Assistants (collectively, Staff) provide professional consultation, research management, and training at any level to support research programs and grant applications. SR commitment to assay development ranges from consults to full responsibility. Through the Cytometry SR, Case CCC members have access to world class, comprehensive microscopy services; the Cytometry SR has successfully implemented a “Full Service” facility that is designed to support clinical trials by providing correlative assays (ie, design, development, optimization, and/or validation, as required by the user). The Cytometry SR provides high quality and essential cell imaging services to multiple investigators across the Case CCC. Use of distant SRs would be inefficient and uncertain, and principle investigators could not complete the aims of NIH grants without access to cytometry and microscopy.

细胞计数和显微镜共享资源（Cytometry SR）是Case Comprehensive的重要资产 癌症中心（案例CCC）成员的研究，说明了科学计划参与的广度， 这反映在使用这种共享资源（SR）的大量研究者中。仅去年一年，就有300多人 调查人员，其中37%是案例CCC成员，占总使用量的40%，来自所有7个案例 CCC程序使用SR。Cytometry SR的使命是支持基本的细胞分析， 转化和临床癌症研究和临床试验。这一多站点SR，联合支持和管理 由凯斯西储大学（CWRU）凯斯综合癌症中心（Case CCC） 医学（SOM），大学医院（UH）和克利夫兰诊所（CC）勒纳研究所（LRI），提供 正式获得昂贵的仪器，以及细胞计数领域的专业知识和援助， 显微镜检查和细胞分选。细胞计数SR的具体目的是： 1.提供维护良好的流式细胞仪、细胞分选仪、显微镜和相关设备。 2.由专业的细胞仪和显微镜人员提供培训和咨询。 3.提供全部或部分基于细胞的检测开发，以支持临床研究者。 在上次审查中，Cytometry SR被评为优秀至非常好。在整个供资周期 （2012-2016年），Cytometry SR为484名注册用户提供了服务，其中132名（27%）是Case CCC 成员，代表所有7个案例CCC计划。 Cytometry SR提供当前维护良好的仪器，在制造商的 规格，并符合环境健康与安全（EHS）的安全指南， 部门（CWRU，CC）和NIH。SR总监、联席总监、经理和助理（统称为员工） 提供任何级别的专业咨询、研究管理和培训，以支持研究项目 和补助金申请。SR对检测开发的承诺范围从咨询到全面负责。通过 Cytometry SR、Case CCC成员可获得世界一流的全面显微镜服务; 细胞测量SR已成功实施“全方位服务”设施，旨在通过以下方式支持临床试验 提供相关分析（即，根据用户要求进行设计、开发、优化和/或验证）。 Cytometry SR为多个研究者提供高质量和必要的细胞成像服务。 案例CCC。使用远距离工作人员代表将是低效和不确定的， 国家卫生研究院赠款的目的，而不使用细胞仪和显微镜。