Rice Bran Microbial Metabolism for Colon Chemoprevention

项目摘要 有必要推进口服膳食补充剂的研究，以包括微生物群落的发展 通过减少结肠炎症来支持结肠健康和新陈代谢。这种方法不应被混淆 与将微生物组合成补充剂的传统方法进行比较，值得注意的是，菌株并没有 起源于微生物群落。初步数据支持一组人类粪便微生物口服给药 对小鼠具有预防结肠炎和预防结肠癌的作用。微生物 口服给小鼠的群落来自于食用富含米糠粉的食物的人 一个月。父项R01奖阐明了米糠饮食改变人类粪便的机制 微生物组以防止结肠癌发生的方式。本补充奖建议 为了从功能上描述作为口服接种正在提供的微生物群落，并将推动 使用计算、综合功能分析的微生物膳食补充剂领域。在这张照片里- 年补充奖期间，我们建议进行全面的元转录和元蛋白质组学 利用创新的工作流程研究结肠健康促进微生物的功能特性 财团。这被认为是在开发和用作以微生物为基础的饮食之前必不可少的 补充剂。我们假设，减少结肠炎症的人类粪便微生物群落 安全性是在作为微生物联盟进行功能健康益处分析时开发的。我们会用凳子 在完成之前和之后从5名女性和5名男性身上收集的微生物群落 饮食干预。本项目选用小鼠粪便微生物联合体口服给药 基于功能功效。利用元转录组学分析细胞内转录活性基因 微生物接种量和代谢活性微生物将与对 冒号。我们还将完成代谢蛋白质组学，以了解哪些蛋白质/酶功能是活跃的 产生了哪些微生物；一种可追溯性，超越了单一微生物的贡献来确定 微生物群落功能。多元组学的应用和集成对于提供一种 对候选口腔“微生物联合体”有完整的机理理解。这个项目也将发展 用于绘制每个微生物联合体的功能代谢网络图的计算工作流程 未来将作为口服膳食补充剂进行测试。

Project Summary There is a need to advance oral dietary supplement research to include development of microbial communities that support colon health and metabolism by reducing colon inflammation. This approach should not be confused with traditional approaches that have combined microbes into a supplement, and notably that the strains did not originate as a microbial community. Preliminary data support that oral delivery of a set of human stool microbes to mice conferred protection against colon inflammation and prevention of colon cancer. The microbial communities that were delivered orally to mice were derived from people that consumed a rice bran rich diet for one month. The parent R01 award is elucidating mechanisms by which a rice bran diet modified the human stool microbiome in a manner that provided protection from colon carcinogenesis. This supplement award proposes to functionally characterize the microbial community that is being delivered as an oral inoculum and will advance the field of microbial based dietary supplements using computational, integrated functional analysis. In this one- year supplement award period, we propose to perform a comprehensive meta-transcriptomic and metaproteomic investigation using an innovative workflow for functional characterization of colon health promoting microbial consortia. This is considered essential prior to development and use as microbial consortia-based dietary supplements. We hypothesize that human stool microbial communities that reduced colon inflammation can be safety developed when analyzed for functional health benefits as a microbial consortia. We will use stool microbial communities that were already collected from 5 females and 5 males before and after completing dietary intervention. The oral delivery of stool microbial consortia to mice were selected for use in this project based on functional efficacy. Employing metatranscriptomics to profile transcriptionally active genes in the microbial inoculum and therefore metabolically active microbes will be correlated with functional effects on the colon. We will also complete metaproteomics to understand what proteins/enzyme functions are actively produced and by which microbes; a traceability that extends beyond single microbial contribution to ascertain microbial community function. The application and integration of multiple meta-omics is essential to provide a complete mechanistic understanding of the candidate oral "microbial consortium". This project will also develop a computational workflow for functional metabolic network mapping of each microbial consortia that will support future testing as an oral dietary supplement.