Rice Bran Microbial Metabolism for Colon Chemoprevention

米糠微生物代谢用于结肠化学预防

• 批准号: 9916241
• 负责人: Komal Raina
• 金额: $ 14.63万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9916241
• 关键词: Attention; Award; Azoxymethane; Bacteria; Biochemical Pathway; Botanicals; Chemoprevention; Chronic Disease; Colon; Colon Carcinoma; Communities; Computer Analysis; Consumption; Data; Data Set; Development; Diet; Dietary Intervention; Disputes; Environment; Enzymes; Feces; Female; Formulation; Future; Gene Expression; Genes; Genetic Transcription; Health; Health Benefit; Health Promotion; Human; Industry; Inflammation; Intestines; Investigation; Knowledge; Metabolic; Metabolic Pathway; Metabolism; Microbe; Mus; Oral; Organism; Parents; Probiotics; Proteins; Proteomics; RNA; Research; Research Support; Rice; Risk; Safety; Sodium Dextran Sulfate; Soil; Supplementation; Taxonomy; Testing; base; colon cancer prevention; colon carcinogenesis; comparative; dietary supplements; fecal microbiome; gut colonization; improved; innovation; male; metaproteomics; metatranscriptome; metatranscriptomics; microbial; microbial community; microbiome; microbiota; microorganism; novel; oral supplementation; plant growth/development; protein metabolite; trait; transcriptomics; wasting; water treatment

Project Summary There is a need to advance oral dietary supplement research to include development of microbial communities that support colon health and metabolism by reducing colon inflammation. This approach should not be confused with traditional approaches that have combined microbes into a supplement, and notably that the strains did not originate as a microbial community. Preliminary data support that oral delivery of a set of human stool microbes to mice conferred protection against colon inflammation and prevention of colon cancer. The microbial communities that were delivered orally to mice were derived from people that consumed a rice bran rich diet for one month. The parent R01 award is elucidating mechanisms by which a rice bran diet modified the human stool microbiome in a manner that provided protection from colon carcinogenesis. This supplement award proposes to functionally characterize the microbial community that is being delivered as an oral inoculum and will advance the field of microbial based dietary supplements using computational, integrated functional analysis. In this one- year supplement award period, we propose to perform a comprehensive meta-transcriptomic and metaproteomic investigation using an innovative workflow for functional characterization of colon health promoting microbial consortia. This is considered essential prior to development and use as microbial consortia-based dietary supplements. We hypothesize that human stool microbial communities that reduced colon inflammation can be safety developed when analyzed for functional health benefits as a microbial consortia. We will use stool microbial communities that were already collected from 5 females and 5 males before and after completing dietary intervention. The oral delivery of stool microbial consortia to mice were selected for use in this project based on functional efficacy. Employing metatranscriptomics to profile transcriptionally active genes in the microbial inoculum and therefore metabolically active microbes will be correlated with functional effects on the colon. We will also complete metaproteomics to understand what proteins/enzyme functions are actively produced and by which microbes; a traceability that extends beyond single microbial contribution to ascertain microbial community function. The application and integration of multiple meta-omics is essential to provide a complete mechanistic understanding of the candidate oral "microbial consortium". This project will also develop a computational workflow for functional metabolic network mapping of each microbial consortia that will support future testing as an oral dietary supplement.

项目摘要 有必要推进口服膳食补充剂的研究，包括微生物群落的发展 通过减少结肠炎症来支持结肠健康和新陈代谢。这种方法不应混淆 传统的方法将微生物结合到补充剂中，值得注意的是， 起源于微生物群落。初步数据支持口服一组人类粪便微生物 给予小鼠保护以对抗结肠炎症和预防结肠癌。微生物 口服给小鼠的群落来自于食用富含米糠的饮食的人， 一个月父母R 01奖阐明了米糠饮食改变人类粪便的机制 微生物组的方式，提供保护结肠癌发生。该补充奖建议 以功能性地表征作为口服接种物递送的微生物群落， 基于微生物的膳食补充剂领域，使用计算，综合功能分析。在这一个- 年补充奖期间，我们建议进行全面的元转录组学和元蛋白质组学 使用创新工作流程进行结肠健康促进微生物功能表征的研究 财团。这被认为是必不可少的开发和使用之前，作为微生物财团为基础的饮食 补充剂.我们假设，减少结肠炎症的人类粪便微生物群落可能是 当作为微生物聚生体分析功能性健康益处时，开发了安全性。我们会用凳子 完成之前和之后已经从5只雌性和5只雄性收集的微生物群落 饮食干预本研究选用小鼠口服粪便微生物菌群的方法 基于功能功效。采用元转录组学分析转录活性基因在 微生物接种物和因此代谢活性微生物将与对微生物的功能效应相关。 结肠我们还将完成元蛋白质组学，以了解哪些蛋白质/酶的功能是积极的 生产和由哪些微生物;可追溯性超出单一微生物的贡献，以确定 微生物群落功能多种元组学的应用和整合对于提供一个 候选口服“微生物聚生体”的完整机理理解。该项目还将开发 用于每个微生物聚生体的功能代谢网络映射的计算工作流程，其将支持 未来作为口服膳食补充剂进行测试。