EFFICACY OF DNA AND PROTEIN CO-IMMUNIZATION PROTOCOL TO PROTECT MACAQUES FROM SHIV INFECTION

HIV疫苗和其他预防策略的开发依赖于在临床前研究中使用非人灵长类动物，以促进有效的AIDS疫苗候选物的开发，并促进有效的局部杀微生物剂和其他预防方式或基于免疫的疗法的开发。NIAID猴疫苗评价单位（SVEU）合同应提供非人灵长类动物资源，主要支持艾滋病疫苗的临床前评价。SVEU进行研究，以支持广泛的研究人员正在开发的疫苗。这些研究补充了NIAID支持的基础疫苗研究和疫苗评估研究，这些研究通过R 01（疫苗启动研究）赠款、HIVRAD（艾滋病毒疫苗研究和设计计划）赠款、IPCAVD（临床前-临床艾滋病疫苗开发综合）赠款和NHP财团赠款资助。SVEU还对公司或其他研究人员提供的候选疫苗进行研究。先前的研究调查了在猕猴中使用HIV或SIV gag和env免疫原的免疫方案，并发现DNA+蛋白质共递送增加了对疫苗的全身和粘膜体液免疫应答的幅度和寿命。研究发现，DNA+蛋白质共递送疫苗方案结合了每个单独疫苗组分的强度，并诱导了强大的细胞应答以及持久的体液免疫。该方案改善了幅度宽度、粘膜传播以及诱导的免疫应答的保护作用。

The development of HIV vaccines and other prevention strategies relies on the use of nonhuman primates in preclinical studies to advance the development of effective AIDS vaccine candidates and to advance development of effective topical microbicides and other prevention modalities or immune-based therapies. The NIAID Simian Vaccine Evaluation Unit (SVEU) contracts shall provide nonhuman primate resources that primarily support preclinical evaluation of AIDS vaccines. The SVEUs conduct studies in support of vaccines being developed by a wide range of investigators. These studies complement NIAID-supported basic vaccine research and vaccine evaluation studies funded through R01 (investigator-initiated research) grants, HIVRAD (HIV Vaccine Research and Design Program) grants, IPCAVD (Integrated Preclinical-Clinical AIDS Vaccine Development) grants, and NHP Consortium awards. The SVEUs also perform studies of candidate vaccines offered by companies or other researchers. Previous studies investigating immunization regimens using HIV or SIV gag and env immunogens in macaques and have found that DNA+protein co-delivery increases the magnitude and longevity of systemic and mucosal humoral immune responses to vaccines. The studies have found that the DNA+protein co-delivery vaccine regimen combines the strength of each individual vaccine component and induces robust cellular responses as well as long-lasting humoral immunity. The regimen improves magnitude breadth, mucosal dissemination as well as protective effects of the induced immune responses.