Analytical Chemistry Core

摘要：核心A 分析核心A是加州大学戴维斯分校超级基金研究中心的一个组成部分。方法 用于检测暴露于环境中有害物质的生物标志物，并评估 这些物质对人类健康的影响。 核心A提供了解决合作研究问题的关键分析支持。复杂 仪器被用来开发所需的色谱和光谱方法的说明 生物标志物。具体而言，核心A提供化合物鉴定并评估化学纯度;鉴定 修复产品、与外源性物质相关的潜在配体和靶向代谢物（项目1、4 为生物传感器验证和环境污染物的免疫化学检测提供了参考方法 提供代谢组学支持（项目1、4和5）。核心A报价 协助有兴趣获得和解释在其他校园服务产生的光谱的研究人员 实验室，如核磁共振和蛋白质组学设施。还提供仪器培训和教育， 核心训练（Core E）。 核心A提供一系列创新服务，重点是分析方法开发。的 核心有3个LC-MS/MS，1个LC-ToF-MS，2个GC-MS和一个加速器质谱仪（AMS）。危险 物质及其代谢物在人类或动物样品中进行测量，并且可以在高浓度下检测到。 灵敏度例如，AMS的检测限为~1阿莫尔14 C，灵敏度是100 000倍 与传统的液体闪烁计数器相比。另一种用于评估危险生物标志物的技术 代谢组学分析是一种高通量的方法，筛选生物体内的代谢物， 样品在这里，一种创新的LC-MS/MS方法用于筛选87种脂质代谢物，以确定 有害物质对调节脂质的影响。 核心A是加州大学戴维斯分校超级基金中心的重要协作组成部分，推动超级基金向前发展 研究项目。该核心推进了评估危险生物标志物所需的分析技术， 物质.核心A的研究人员继续致力于扩大代谢组学平台， 提供更高的灵敏度和更高的吞吐量。AMS吞吐量也在增加，从而允许高效的 样品分析通过提供创新和先进的技术，该核心是研究的重要组成部分 程序.它产生的解决方案周围的环境污染的危险 物质，并询问这些物质对人类健康构成的风险。

ABSTRACT: CORE A The Analytical Core A functions as an integral part of the Superfund Research Center at UC Davis. Methods are developed to detect biomarkers of exposure to hazardous substances in the environment, and to assess the impact these substances have on human health. Core A provides the analytical support critical to solving collaborative research problems. Sophisticated instrumentation is used to develop chromatographic and spectrometric methods necessary for the elucidation of biomarkers. Specifically, Core A provides compound identification and assesses chemical purity; identifies remediation products, potential ligands associated with xenobiotics, and targeted metabolites (Projects 1, 4 and 5); provides reference methods for biosensor validation and immunochemical detection of environmental substances (Projects 2 and 3); and provides metabolomics support (Projects 1, 4 and 5). Core A offers assistance to investigators interested in obtaining and interpreting spectra generated at other campus service laboratories such as the NMR and proteomic facility. Instrument training and education is also offered to investigators in conjunction with the Training Core (Core E). A range of innovative services is offered by Core A, with emphasis on analytical method development. The Core has 3 LC-MS/MSs, 1 LC-ToF-MS, 2 GC-MSs, and an Accelerator Mass Spectrometer (AMS). Hazardous substances and their metabolites are measured in human or animal samples, and can be detected with high sensitivity. For example, the AMS has a detection limit of ~1 amol 14C, and is 100 000 times more sensitive than the traditional liquid scintillation counter. Another technique used to assess biomarkers of hazardous substances is metabolomics analysis, a high throughput method that screens for metabolites within biological samples. Here, an innovative LC-MS/MS method is used to screen for 87 lipid metabolites to determine the consequence of hazardous substances on regulatory lipids. Core A is a significant collaborative component of the UC Davis Superfund Center, driving forward Superfund research projects. This Core advances the analytical techniques required to assess biomarkers of hazardous substances. Researchers within Core A continue to work on expanding the metabolomics platform in order to provide more sensitivity and higher throughput. The AMS throughput is also increasing, allowing for efficient sample analysis. By providing innovative and advanced technology, this Core is a valued part of the research program. It generates solutions to problems surrounding the environmental contamination of hazardous substances, and interrogates the risk these substances pose on human health.