开发用于长期表达 eCD4-Ig 的 AAV 载体-猫眼课题宝

开发用于长期表达 eCD4-Ig 的 AAV 载体

批准号：

9979748

负责人：

MICHAEL DAVID ALPERT

金额：

$99.94万

依托单位：

EMMUNE, INC

依托单位国家：

美国

项目类别：

财政年份：

2017

资助国家：

美国

项目状态：

已结题

起止时间：

2017-08-01 至 2021-07-31

来源：

https://reporter.nih.gov/project-details/9979748

关键词：

Affinity Antibodies Antibody Formation Antibody Response Antigen-Presenting Cells Antiviral Agents Binding CCR5 gene CMV promoter Dependovirus Development Dose Engineering Enhancers Enzymes Epitopes Exhibits Gene Expression Glycoproteins Grant HIV HIV-1 Healthcare Systems Human T-lymphotropic virus 1 Immune response Immunoglobulin Constant Region Immunoglobulin Somatic Hypermutation Immunologics In Vitro Infection Innate Immune Response Internal Ribosome Entry Site Intramuscular Injections Intravenous Introns Laboratory Animals Life Longevity Macaca Macaca mulatta Methylation Muscle Muscle Cells Nature Patients Peptide Hydrolases Plasma Polyadenylation Post-Translational Protein Processing Primates Production Research Risk Simian virus 40 Site Structure T cell response Testing Time Transgenes Viral Virus Receptors adeno-associated viral vector base cellular transduction cost design expression vector fitness gene transfer vector global health immunogenic immunogenicity improved innovation neutralizing antibody novel pandemic disease peptidomimetics prevent promoter protein-tyrosine sulfotransferase 2 public health relevance receptor simian human immunodeficiency virus synthetic antibodies transcription factor transgene expression vector vector genome

项目摘要

项目成果

MICHAEL DAVID ALPERT的其他基金

相似基金

相关文献

中文摘要

翻译

点击翻译按钮获取中文摘要

英文摘要

ABSTRACT / SUMMARY / PROJECT DESCRIPTION A single intramuscular injection of an adeno-associated virus (AAV) vector that expresses eCD4-Ig has the potential to treat or prevent HIV-1 infection for a lifetime. eCD4-Ig is a synthetic antibody or “immunoadhesin” constructed by fusing parts of the viral receptor (CD4) and coreceptor (CCR5) to the constant region of an antibody. Because eCD4-Ig binds the HIV-1 envelope glycoprotein (Env) through the same contacts used by Env to recognize its receptor and coreceptor, eCD4-Ig neutralizes 100% of HIV-1 isolates and presents fundamental barriers to viral escape. We have previously shown that rhesus macaques inoculated with AAV expressing eCD4-Ig exhibited sterilizing protection against high-dose intravenous challenges with simian- human immunodeficiency virus (SHIV). Now, we propose several practical innovations designed to maximize the concentrations of eCD4-Ig in plasma that are sustained over the long term by AAV-transduced muscle.

期刊论文(0)

专著(0)

科研奖励(0)

会议论文