Structural Biology (SB)

项目摘要/摘要：结构生物学设施 结构生物学(SB)设施的总体目标是应用三维结构可视化 重要的癌症相关生物大分子的分析技术。这样的结构数据可以 提供疾病机制和驱动力研究的基本知识。活性中心和活性中心的原子可视化 蛋白质之间的相互作用可以指导新的治疗药物的合成。SB的联席董事和工作人员 设施使这些复杂的、高技术的生物物理方法的成员很容易获得 癌症中心。SB基金是十年前通过内布拉斯加州研究中心提供的资金发起的 倡议(NRI)和负责研究的副校长。SB设施有五个免费实验室 成分：蛋白质表达和纯化(PrEP)、晶体筛选和生长(CSG)、X射线 结晶学数据采集、小角X射线散射(SAXS)数据采集和核磁 磁共振(核磁共振)数据采集。PrEP组件是该设施的中心部分，用于准备样品 用于CSG(以及随后的X射线结晶学)、SAXS和/或核磁共振。SB工厂运行的是固体 “协作性”商业计划，包括生产样品和/或使用 工具以及将费用纳入协作性赠款提案。由SB提供的数据 该基金帮助资助了许多外部赠款提案，包括来自NIH、NSF和NASA的提案。 癌症研究中许多合作的、同行评议的出版物以及受邀的评论文章都有 是由SB设施造成的。

Project Summary/Abstract: Structural Biology Facility The overall goal of the Structural Biology (SB) Facility is to apply three-dimensional structural visualization techniques to the analysis of important cancer-related biological macromolecules. Such structural data can provide basic knowledge of disease mechanism and drive research. Atomic visualization of active sites and protein-protein interactions can direct the synthesis of novel therapeutics. The co-directors and staff of the SB Facility make access to these sophisticated, highly-technical biophysical methods easy for members of the cancer center. The SB Facility was initiated ten years ago, through funding from the Nebraska Research Initiative (NRI) and the Vice Chancellor for Research. The SB Facility has five complimentary laboratory components: Protein expression and purification (PrEP), crystal screening and growth (CSG), X-ray crystallography data collection, small-angle X-ray scattering (SAXS) data collection and nuclear magnetic resonance (NMR) data collection. The PrEP component is a central part of the facility and prepares samples for CSG (and subsequently X-ray crystallography), SAXS and/or NMR. The SB Facility is run with a solid “collaborative” business plan that includes fees collected upon production of samples and/or use of the instrumentation, as well as, inclusion of costs in collaborative grant proposals. Data provided from the SB Facility has helped fund numerous external grant proposals including those from NIH, NSF and NASA. Numerous collaborative, peer-reviewed publications in cancer research as well as invited review articles have resulted from the SB Facility.