Structural Biology (SB)

结构生物学（SB）

• 批准号: 9981653
• 负责人: Gloria Borgstahl
• 金额: $ 11.44万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9981653
• 关键词: Active Sites; Applications Grants; Biological; Businesses; Cancer Center; Cancer Center Support Grant; Crystallization; Data; Data Collection; Disease; Fees; Funding; Goals; Growth; Knowledge; Laboratories; Malignant Neoplasms; Nebraska; Nuclear Magnetic Resonance; Peer Review; Production; Publications; Research; Roentgen Rays; Running; Sampling; Solid; Structure; Techniques; United States National Aeronautics and Space Administration; United States National Institutes of Health; Visualization; X-Ray Crystallography; anticancer research; biophysical techniques; cost; instrumentation; macromolecule; member; novel therapeutics; protein expression; protein protein interaction; protein purification; screening; structural biology; structured data; three dimensional structure

Project Summary/Abstract: Structural Biology Facility The overall goal of the Structural Biology (SB) Facility is to apply three-dimensional structural visualization techniques to the analysis of important cancer-related biological macromolecules. Such structural data can provide basic knowledge of disease mechanism and drive research. Atomic visualization of active sites and protein-protein interactions can direct the synthesis of novel therapeutics. The co-directors and staff of the SB Facility make access to these sophisticated, highly-technical biophysical methods easy for members of the cancer center. The SB Facility was initiated ten years ago, through funding from the Nebraska Research Initiative (NRI) and the Vice Chancellor for Research. The SB Facility has five complimentary laboratory components: Protein expression and purification (PrEP), crystal screening and growth (CSG), X-ray crystallography data collection, small-angle X-ray scattering (SAXS) data collection and nuclear magnetic resonance (NMR) data collection. The PrEP component is a central part of the facility and prepares samples for CSG (and subsequently X-ray crystallography), SAXS and/or NMR. The SB Facility is run with a solid “collaborative” business plan that includes fees collected upon production of samples and/or use of the instrumentation, as well as, inclusion of costs in collaborative grant proposals. Data provided from the SB Facility has helped fund numerous external grant proposals including those from NIH, NSF and NASA. Numerous collaborative, peer-reviewed publications in cancer research as well as invited review articles have resulted from the SB Facility.

项目概要/摘要：结构生物学设施 结构生物学（SB）设施的总体目标是应用三维结构可视化 技术分析重要的癌症相关的生物大分子。这种结构数据可以 提供疾病机制的基础知识，推动研究。活性位点的原子可视化， 蛋白质-蛋白质相互作用可以指导新治疗剂的合成。SB的联合董事和工作人员 设施使这些复杂的，高技术的生物物理方法容易为成员 癌症中心SB基金于10年前启动，由内布拉斯加州研究中心提供资金。 倡议（NRI）和副校长的研究。SB设施有五个免费实验室 组成部分：蛋白表达和纯化（PrEP），晶体筛选和生长（CSG），X射线 晶体学数据收集、小角X射线散射（SAXS）数据收集和核磁共振数据收集 共振（NMR）数据收集。PrEP组件是该设施的核心部分，用于制备样本 用于CSG（以及随后的X射线晶体学）、SAXS和/或NMR。SB设施使用固体 “合作”商业计划，包括在制作样品和/或使用 工具，以及在合作赠款提案中纳入成本。SB提供的数据 该基金已帮助资助许多外部赠款提案，包括来自NIH，NSF和NASA的提案。 许多合作，同行评议的出版物在癌症研究以及邀请审查的文章， 源于SB设施。