The BCL-2 family controls stem cell identity by regulating mitochondrial dynamics and priming

总结 干细胞领域的研究传统上集中于理解关键的转录因子， 提供多能细胞身份。然而，对其他关键的非转录因子知之甚少。 控制干细胞身份和谱系定型的信号网络。我们的初步研究表明 BCL-2蛋白家族在维持细胞特性方面发挥着积极作用， 独立于其在线粒体外透化中的作用。我们将采用多学科方法 结合分子生物学、生物化学、遗传学和细胞生物学来解决基本问题 例如：BCL-2家族在维持一个片段化的 干细胞中的线粒体网络与其在细胞凋亡中的作用无关？是线粒体动力学 通过BCL-2家族介导细胞重编程的需要？信号是什么 参与将信息从线粒体翻译到细胞核， 调节细胞命运我们计划联合收割机结合我们在线粒体和干细胞生物学方面的专业知识， 本发明提供了寻求这些问题的答案并揭示BCL-2家族在肿瘤中的新功能的最新方法。 干细胞身份和承诺。

Summary Research in the stem cell field has traditionally focused on understanding key transcriptional factors that provide pluripotent cell identity. However, much less is known about other critical non-transcriptional signaling networks that govern stem cell identity and lineage commitment. Our preliminary studies suggest an emerging landscape in which the BCL-2 family of proteins has an active role in maintaining cell identity independently of its role in mitochondrial outer permeabilization. We will employ a multidisciplinary approach combining molecular biology, biochemistry, genetics, and cell biology to address fundamental questions such as: What are the signaling events involved in the role of BCL-2 family maintaining a fragmented mitochondrial network in stem cells independently of its role in apoptosis? Are mitochondrial dynamics mediated through the BCL-2 family a requirement for cellular reprogramming? What are the signaling mechanisms involved in translating the information from the mitochondria into the nucleus to ultimately modulate cell fate? We plan to combine our expertise in mitochondrial and stem cell biology with state-of- the-art approaches to seek answers to these questions and reveal novel functions of the BCL-2 family in stem cell identity and commitment.