Live Cell Fluorescent Imaging System

项目总结/摘要 维克森林大学的一个重点发展领域是细胞和分子的研究 微生物生物体方面。这些研究团队正在追求的新研究目标将 揭示与人类健康直接相关的微生物内部的详细生化机制 和疾病状态。显微镜是这里概述的所有研究的关键工具， 研究者们共同提出了强调形态学数据测量的建议， 卓越品质的荧光和明场图像。虽然维克森林的显微成像 核心设施一直是生物学系的优势历史领域，目前的需求是 在特定功能和可用性方面， 成像时间这里要求的Leica Thunder活细胞成像仪系统代表了一种理想的 平台，以满足本提案中概述的定向研究目标和计划。它将扩大我们的 通过提供高速荧光采集系统， 组织成像条件，并将提供一种新的方法，自动化大样本领土 可视化和详细的图像采集。除了提供设备，这将是至关重要的， 为了开展此处概述的指导活动，该系统将作为扩大我们的 现存的技术框架，刺激和支持生物学的微观研究， 我们地区的人类健康。

PROJECT SUMMARY/ABSTRACT A developing area of focus for Wake Forest University is the investigation of cellular and molecular aspects of microbial organisms. Novel research goals being pursued by these study teams will uncover detailed biochemical mechanisms within microbes that have direct relevance to human health and disease states. Microscopy represents a critical tool for all the studies outlined here with investigators collectively proposing emphasis on morphologic data measures that require acquisition of exceptional quality fluorescent and brightfield images. While the Wake Forest Microscopic Imaging Core Facility has been an historic area of strength for the Department of Biology, current needs are exceeding the available equipment capabilities in terms of both specific functionality and available imaging time. The Leica Thunder Live Cell Imager system requested here represents an ideal platform for meeting the directed research goals and plans outlined in this proposal. It will expand our existing capabilities by providing a high-speed fluorescence acquisition system with optimized living tissue imaging conditions, and will provide a new approach for automated large sample territory visualization and detailed image acquisition. In addition to providing equipment that will be crucial to carry out the directed activities outlined here, this system will serve as a key addition to broaden our extant technical framework that stimulates and supports microscopic investigations of biology and human health in our region.