Live Cell Fluorescent Imaging System

项目摘要/摘要 维克森林大学正在发展的一个重点领域是细胞和分子研究 微生物的各个方面。这些研究团队正在追求的新研究目标将 揭示微生物体内与人类健康直接相关的详细生化机制 和疾病状态。显微镜是这里概述的所有研究的关键工具 研究人员集体提出强调需要获取的形态数据测量 超高质量的荧光和亮场图像。而维克森林的显微成像 核心设施一直是生物系历史上的强项，目前的需求是 在特定功能和可用方面都超过可用的设备能力 成像时间。在此要求的徕卡雷霆活细胞成像仪系统代表了理想的 实现本提案中所述的定向研究目标和计划的平台。它将扩大我们的 通过提供优化生活的高速荧光采集系统来实现现有功能 组织成像条件，将为自动化大样本领域提供一种新的方法 可视化和详细的图像获取。除了提供关键的设备， 执行此处列出的定向活动，该系统将作为一个关键的补充来扩大我们的 现有的技术框架，刺激和支持对生物学和 我们地区的人类健康。

PROJECT SUMMARY/ABSTRACT A developing area of focus for Wake Forest University is the investigation of cellular and molecular aspects of microbial organisms. Novel research goals being pursued by these study teams will uncover detailed biochemical mechanisms within microbes that have direct relevance to human health and disease states. Microscopy represents a critical tool for all the studies outlined here with investigators collectively proposing emphasis on morphologic data measures that require acquisition of exceptional quality fluorescent and brightfield images. While the Wake Forest Microscopic Imaging Core Facility has been an historic area of strength for the Department of Biology, current needs are exceeding the available equipment capabilities in terms of both specific functionality and available imaging time. The Leica Thunder Live Cell Imager system requested here represents an ideal platform for meeting the directed research goals and plans outlined in this proposal. It will expand our existing capabilities by providing a high-speed fluorescence acquisition system with optimized living tissue imaging conditions, and will provide a new approach for automated large sample territory visualization and detailed image acquisition. In addition to providing equipment that will be crucial to carry out the directed activities outlined here, this system will serve as a key addition to broaden our extant technical framework that stimulates and supports microscopic investigations of biology and human health in our region.