Application of single-cell trajectory analysis in single-cell transcriptomics to elucidate the biology of lung pericytes in injury, repair, and regeneration

摘要 周细胞与许多器官的炎症和纤维化有关。数据来自 激活培养的肺周细胞转录转录分析显示多个 生物途径包括炎症、血管生成过程和细胞迁移。目标是 本项目的目的是了解肺周细胞在整个损伤过程中的功能可塑性。 肺修复手术。我们感兴趣的是肺周细胞如何通过 血管生成与肌成纤维细胞分化。在目标1中，我们建议使用最先进的 基于单细胞转录数据的单细胞轨迹分析用于描述肺细胞命运 周细胞从急性肺损伤到纤维化。在目标2中，我们建议研究选定的基质 纤维化过程中来源于周细胞和非周细胞来源的肌成纤维细胞的亚群 研究它们在体外的功能差异。

ABSTRACT Pericytes have been implicated in inflammation and fibrosis in a number of organs. Data from transcriptomic analyses of activated cultured lung pericytes reveal upregulation of multiple biological pathways including inflammation, angiogenic processes, and cell migration. The goal of this project is to understand the functional plasticity of lung pericytes throughout injury and in lung repair. We are interested in how lung pericytes modulate lung repair in lung fibrosis through angiogenesis and myofibroblast differentiation. In Aim 1, we propose to use state-of-the-art single cell trajectory analysis on single-cell transcriptomic data to describe the cell fate of lung pericytes from acute lung injury to fibrosis. In Aim 2, we propose to study selected stromal subpopulations derived from pericytes and non-pericyte-derived myofibroblasts during fibrosis to examine their functional differences ex vivo.