Mechanistic and Bioinformatics Core

机制和生物信息学核心

• 批准号: 10642595
• 负责人: Jordi Ochando
• 金额: $ 48.27万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10642595
• 关键词: Address; Animals; Bioinformatics; Biological Assay; Biometry; Cells; Cellular Assay; Clinical; Communication; Computer software; Custom; Data; Data Analyses; Data Set; Data Storage and Retrieval; Ensure; Epigenetic Process; Equipment and supply inventories; Experimental Designs; Flow Cytometry; Gene Expression; Genes; Head; Human; Image; Immune; Immunity; Immunologist; In Vitro; Infrastructure; International; Investigation; Laboratories; Laboratory Research; Medicine; Metabolic Pathway; Metabolism; Mus; Netherlands; Organ Transplant Research; Procedures; Protocols documentation; Records; Reproducibility; Research Personnel; Sampling; Standardization; System; Testing; Therapeutic; Training; Transplantation; data exploration; data handling; data integration; data management; data pipeline; epigenome; experience; experimental study; human error; in silico; in vivo; large datasets; medical schools; monocyte; multiple data types; multiple omics; next generation sequencing; professor; programs; success; therapeutic target; transcription factor; transcriptomics

SUMMARY – CORE C Trained immunity is characterized by pronounced changes in the epigenome, gene expression, metabolism, and cell dynamics. The investigators associated with this Program Project will perform comprehensive studies on trained immunity in the context of transplantation. To safeguard the appropriate implementation of trained immunity assays across the different projects, we have established a Mechanistic and Bioinformatics Core (Core C). Core C will operate at Mount Sinai and be directed by Dr. Jordi Ochando, an internationally recognized transplant immunologist and one of the founders of trained immunity in organ transplantation research. The Core team will provide expertise and in-house optimized protocols to profile immune cells and biologically validate promising therapeutic targets. Importantly, this Core will also safeguard bioinformatics and biostatistics analyses and general data management and storage. Lastly, Core C will implement and manage Labguru, a laboratory management system used to track samples, record, and share protocols and data, and keep inventory.

摘要-核心C 训练免疫的特征在于表观基因组、基因表达、代谢和免疫应答的显著变化。 细胞动力学与本计划项目相关的研究人员将对以下方面进行全面研究： 在移植的背景下训练免疫力。为保障培训的适当实施， 在不同项目的免疫测定，我们已经建立了一个机制和生物信息学核心 (Core C）。核心C将在西奈山运作，由国际知名的Jordi Ochando博士指导。 公认的移植免疫学家和器官移植免疫训练的创始人之一 research.核心团队将提供专业知识和内部优化方案，以分析免疫细胞， 生物学验证有前景的治疗靶点。重要的是，该核心还将保护生物信息学， 生物统计分析和一般数据管理和存储。最后，核心C将实施和管理 Labguru是一个实验室管理系统，用于跟踪样本，记录和共享协议和数据， 保持库存。