Protein Production and Characterization Core

蛋白质生产和表征核心

• 批准号: 10631167
• 负责人: Philip Charles Bourne
• 金额: $ 28.54万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10631167
• 关键词: Advisory Committees; Applications Grants; Award; Biological Sciences; Centers of Research Excellence; Collaborations; Communities; Core Facility; Cryoelectron Microscopy; Crystallization; Data; Educational workshop; Equipment; Faculty; Fee-for-Service Plans; Fostering; Grant; Individual; Laboratories; Laboratory Research; Learning Module; Maintenance; Mass Spectrum Analysis; Methodology; Microscopy; Molecular; Names; Oklahoma; Peer Review; Phase; Production; Proteins; Proteomics; Protocols documentation; Publications; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Scientist; Services; Structure; Techniques; Technology; Training; Universities; Work; base; biophysical properties; cost; graduate student; innovation; instrumentation; macromolecule; metabolomics; next generation; outreach; posters; protein expression; protein purification; protein structure; structural biology; symposium

Project Summary (Protein Production and Characterization Core) The Protein Production and Characterization Core (PPCC) serves as research support for the Oklahoma COBRE in Structural Biology (OCSB) and the greater life science research community. It is a state-of-the-art facility for the expression, purification, and biophysical characterization of proteins for researchers. The PPCC produces high quality proteins in a reliable and efficient manner on a fee-for-service basis and provides hands-on training and expert advice in all aspects of protein expression, purification, and characterization available in the facility. The PPCC staff consists of the Core Director Dr. Philip Bourne and a Graduate Student Assistant. The PPCC is guided by an advisory committee comprised of three faculty users. The PPCC works closely with investigators and the OCSB-supported Biomolecular Structure Core (BSC) to produce homogeneous, highly purified protein for crystallization trials and other life science research needs. The facility is equipped with instrumentation frequently not found within individual research laboratories due to their cost and maintenance. Recently, instrumentation for the biophysical characterization of macromolecules was added to the facility and fostered the expansion of services and resulting increase in the user base. During the proposed Phase 3 of the OCSB, the PPCC will work with the BSC, the OU Mass Spectrometry, Proteomics & Metabolomics Core, and the cryo-EM component of the OU Samuel Roberts Noble Microscopy Laboratory to support researchers in their protein needs for structural biology methodologies. Being a dynamic resource for the life science community will continue to grow the user base and, together with the support of the University, provide for the long-term sustainability of this core facility.

项目摘要（蛋白质生产和表征核心） 蛋白质生产和表征核心 (PPCC) 为俄克拉荷马州 COBRE 提供研究支持 结构生物学（OCSB）和更大的生命科学研究界。这是一个最先进的设施 为研究人员提供蛋白质的表达、纯化和生物物理表征。 PPCC 生产 以可靠、高效的方式在收费服务的基础上提供高质量蛋白质，并提供实践培训 以及设施中提供的蛋白质表达、纯化和表征各个方面的专家建议。 PPCC 工作人员由核心主任 Philip Bourne 博士和研究生助理组成。政协是 由三名教员用户组成的咨询委员会指导。 PPCC 与研究人员和 OCSB 支持的生物分子结构核心 (BSC) 密切合作， 生产均质、高度纯化的蛋白质，用于结晶试验和其他生命科学研究需求。这 设施配备了在个别研究实验室中经常找不到的仪器，因为它们 成本和维护。最近，用于大分子生物物理表征的仪器被开发出来。 增加了设施并促进了服务的扩展，从而增加了用户群。 在拟议的 OCSB 第 3 阶段期间，PPCC 将与 BSC、OU 质谱、 蛋白质组学和代谢组学核心，以及 OU Samuel Roberts Noble 显微镜的冷冻电镜组件 实验室支持研究人员满足结构生物学方法的蛋白质需求。成为一个动态的 生命科学界的资源将继续扩大用户群，并在 大学，确保这一核心设施的长期可持续性。