Protein Production and Characterization Core

蛋白质生产和表征核心

• 批准号: 10631167
• 负责人: Philip Charles Bourne
• 金额: $ 28.54万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10631167
• 关键词: Advisory Committees; Applications Grants; Award; Biological Sciences; Centers of Research Excellence; Collaborations; Communities; Core Facility; Cryoelectron Microscopy; Crystallization; Data; Educational workshop; Equipment; Faculty; Fee-for-Service Plans; Fostering; Grant; Individual; Laboratories; Laboratory Research; Learning Module; Maintenance; Mass Spectrum Analysis; Methodology; Microscopy; Molecular; Names; Oklahoma; Peer Review; Phase; Production; Proteins; Proteomics; Protocols documentation; Publications; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Scientist; Services; Structure; Techniques; Technology; Training; Universities; Work; base; biophysical properties; cost; graduate student; innovation; instrumentation; macromolecule; metabolomics; next generation; outreach; posters; protein expression; protein purification; protein structure; structural biology; symposium

Project Summary (Protein Production and Characterization Core) The Protein Production and Characterization Core (PPCC) serves as research support for the Oklahoma COBRE in Structural Biology (OCSB) and the greater life science research community. It is a state-of-the-art facility for the expression, purification, and biophysical characterization of proteins for researchers. The PPCC produces high quality proteins in a reliable and efficient manner on a fee-for-service basis and provides hands-on training and expert advice in all aspects of protein expression, purification, and characterization available in the facility. The PPCC staff consists of the Core Director Dr. Philip Bourne and a Graduate Student Assistant. The PPCC is guided by an advisory committee comprised of three faculty users. The PPCC works closely with investigators and the OCSB-supported Biomolecular Structure Core (BSC) to produce homogeneous, highly purified protein for crystallization trials and other life science research needs. The facility is equipped with instrumentation frequently not found within individual research laboratories due to their cost and maintenance. Recently, instrumentation for the biophysical characterization of macromolecules was added to the facility and fostered the expansion of services and resulting increase in the user base. During the proposed Phase 3 of the OCSB, the PPCC will work with the BSC, the OU Mass Spectrometry, Proteomics & Metabolomics Core, and the cryo-EM component of the OU Samuel Roberts Noble Microscopy Laboratory to support researchers in their protein needs for structural biology methodologies. Being a dynamic resource for the life science community will continue to grow the user base and, together with the support of the University, provide for the long-term sustainability of this core facility.

项目概要（蛋白质生产和表征核心） 蛋白质生产和表征核心（PPCC）为俄克拉荷马州COBRE提供研究支持 在结构生物学（OCSB）和更大的生命科学研究社区。这是最先进的设施， 为研究人员提供蛋白质的表达、纯化和生物物理特性。PPCC生产 高品质的蛋白质以可靠和有效的方式在收费服务的基础上，并提供动手培训 以及在设施中可用的蛋白质表达、纯化和表征的所有方面的专家建议。 PPCC的工作人员包括核心主任菲利普·伯恩博士和一名研究生助理。PPCC是 由一个由三名教师用户组成的咨询委员会指导。 PPCC与研究人员和OCSB支持的生物分子结构核心（BSC）密切合作， 生产均匀的、高度纯化的蛋白质，用于结晶试验和其他生命科学研究需求。的 该设施配备的仪器往往没有发现在个别研究实验室，由于他们的 成本和维护。最近，用于大分子的生物物理表征的仪器得到了广泛的应用。 增加了设施，促进了服务的扩展，从而增加了用户群。 在拟议的OCSB第三阶段，PPCC将与BSC、质谱仪、 蛋白质组学和代谢组学核心，以及OU Samuel Roberts Noble显微镜的冷冻EM组件 实验室，以支持研究人员在他们的蛋白质需求的结构生物学方法。是动态 生命科学界的资源将继续扩大用户群，并与 大学，提供这一核心设施的长期可持续性。