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Rapid NGS Method for Mapping of the Epitranscriptome

表观转录组图谱快速 NGS 方法

基本信息

批准号：
10697296
负责人：
Gudrun Stengel
金额：
$ 110.74万
依托单位：
ALIDA BIOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-13 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10697296
关键词：
Acceleration Active Sites Alternative Splicing Antibodies Architecture Bar Codes Benchmarking Binding Biological Biological Assay Biological Sciences Cells Chemicals Chimeric Proteins Clinical Code Custom DNA Data Data Set Detection Development Disease Disease Progression Drug Targeting Drug resistance Elements Engineering Environmental Risk Factor Enzymes Evaluation Exhibits Feedback Funding Genetic Code Goals Grant Health Heteroduplex DNA Human Human Cell Line Individual Libraries Location Malignant Neoplasms Maps Marketing Measures Messenger RNA Methods Modification Monitor Mutagenesis Needle biopsy procedure Nucleotides Pathway interactions Performance Phase Phenotype Play Procedures Process Proteins Protocols documentation Publishing Questionnaires RNA RNA Degradation Reader Reading Reagent Reproducibility Reverse Transcription Ribosomal RNA Role Running Sampling Science Site Small Business Innovation Research Grant Specificity Standardization Structure Technology Testing Time Tn5 transposase Transfer RNA Translation Initiation Translations Transposase Validation Variant Virus Diseases analysis pipeline analytical method commercialization design detection assay detection limit drug development epitranscriptome epitranscriptomics improved interest molecular recognition nano next generation sequencing novel strategies posttranscriptional prototype rational design trafficking transcriptome tumor progression usability user-friendly

项目摘要

Project Summary/Abstract Epitranscritomics is the study of RNA modifications, which include more than 170 naturally occurring chemical alternations to the nucleotides. More than 60 are found in human RNA of all types: mRNA, tRNA, rRNA, lncRNA, and the others. These modifications are dynamic; their global quantities change in development and during disease progression. They are installed by writer enzymes, read by reader proteins and removed by eraser enzymes, and they have an intrinsic capacity to alter RNA structure and dynamics. They influence translation initiation and termination, translation fidelity, alternative splicing, trafficking between cellular compartments, and regulate RNA degradation. RNA reader, writer and eraser proteins are promising drug targets of high current interest to pharma. In this project, we will develop and commercialize a new approach to detecting, identifying, and mapping, RNA modifications in a multiplex and with high sensitivity—suitable for clinical samples (e.g. needle biopsies, FFPE samples) in which only sub-nanogram quantities of RNA may be available. This technology will be significant because it will provide the first commercial method for profiling and correlating changes of multiple RNA modification types across the entire transcriptome using low sample input.

项目总结/摘要表位转录组学是研究RNA修饰的一门科学，包括170多种天然存在的化学修饰。对核苷酸的改变。在人类所有类型的RNA中发现了60多种：mRNA，tRNA，rRNA，lncRNA，还有其他人这些修改是动态的;它们的整体数量在发展和期间发生变化疾病进展。它们由写入酶安装，由读取蛋白读取，并由擦除器移除它们具有改变RNA结构和动力学的内在能力。影响翻译起始和终止、翻译保真度、可变剪接、细胞区室之间的运输，以及调节RNA降解。RNA阅读器、写入器和擦除器蛋白是一种很有前途的高电流药物靶点对制药感兴趣。在这个项目中，我们将开发和商业化一种新的方法来检测，识别，和映射，RNA修饰的多重和高灵敏度-适用于临床样品（例如，针活检，FFPE样品），其中仅可获得亚纳克量的RNA。这这项技术将具有重要意义，因为它将提供第一种用于分析和关联的商业方法使用低样品输入，在整个转录组中的多种RNA修饰类型的变化。