Deciphering the mechanisms mediating the dichotomous effects of fever on brain function and behavior

动物护理和使用委员会于2020年7月29日批准了“解密介导发热对脑功能和行为的二分效应的机制”的动物方案。到目前为止，我们已经获得并开始培育10种不同的ASD和发热诱导癫痫发作的真实模型中的6种。其余的线路将在今后两个月内生成和收到。 必要的设备和试剂： a）诱导和检测发热和B）量化癫痫发作和ASD相关行为：例如社会交流缺陷、感觉异常和重复行为。 c）测量发烧对大脑神经元活动的影响。 初步的数据已被记录和分析，成功地揭示了正常的神经回路机制，在暴露于发热温度时，在发展中的皮层。未来的实验将描述与热性惊厥和自闭症谱系障碍相关的基因突变如何破坏或改变大脑中这些正常的发热温度反应机制。

The animal protocol for "Deciphering the mechanisms mediating the dichotomous effects of fever on brain function and behavior" was approved by the Animal Care and Use Committee on 07/29/2020. Thus far, we have obtained and initiated breeding of 6 out of 10 different bonafide models of ASD and fever-induced seizures. The remaining lines will be generated and received within the next two months. The necessary equipment and reagents for: a) inducing and detecting fevers and b) quantifying seizures and ASD-related behaviors: such as socio-communication deficits, sensory abnormalities and repetitive behaviors, have been obtained. c) measuring the effects of fever on the activity of neurons in the brain has been achieved. Preliminary data has been recorded and analyzed, to successfully reveal the normal neural circuit mechanisms that are engaged in the developing cortex upon exposure to febrile temperatures. Future experiments will delineate how genetic mutations linked to febrile seizures and Autism Spectrum Disorder disrupt or modify these normal febrile temperature responsive mechanisms in the brain.