Examining Associations between the Oral Microbiota, Neuroinflammation, and Binge Drinking in Adolescents

检查青少年口腔微生物群、神经炎症和酗酒之间的关联

• 批准号: 10679789
• 负责人: Brittney Browning
• 金额: $ 4.51万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10679789
• 关键词: 16S ribosomal RNA sequencing; 21 year old; Address; Adolescence; Adolescent; Adult; Affect; Age; Alcohol abuse; Alcohol consumption; Alcohols; Anterior; Area; Astrocytes; Brain; Brain region; Cells; Choline; Clinical; Clinical Trials; Data; Data Analyses; Data Collection; Development; Diagnosis; Diagnostic; Eligibility Determination; Enzyme-Linked Immunosorbent Assay; Epithelial Cells; Foundations; Funding; Gingiva; Goals; Human; Immune; Immune system; Individual; Inflammatory; Interleukin-1 beta; Interleukin-6; Intervention; Knowledge; Life; Magnetic Resonance Spectroscopy; Maintenance; Measures; Mediating; Meta-Analysis; Methods; Microbe; Microglia; Neurobiology; Neuroglia; Neurosciences; Oral; Participant; Peripheral; Physiological Processes; Play; Population; Positioning Attribute; Postdoctoral Fellow; Predisposition; Prevention strategy; Protons; Research; Risk Factors; Role; Saliva; Sampling; Secure; Substance Use Disorder; TNF gene; Training; United States National Institutes of Health; Youth; adolescent binge drinking; alcohol effect; alcohol use disorder; alcohol use initiation; binge drinker; binge drinking; career development; cingulate cortex; cohort; comparison control; critical period; cytokine; disorder prevention; early alcohol use; experience; gut microbiota; hands on research; in vivo; inflammatory marker; interest; microbial; microbial composition; microbiome; microbiome alteration; microbiome research; microbiota; myoinositol; neural; neurodevelopment; neuroimaging; neuroinflammation; novel; oral microbial community; parent grant; response biomarker; skills; training opportunity; translational scientist; translational study; treatment response; underage drinking

PROJECT SUMMARY/ABSTRACT The proposed application responds to the need for a better understanding of the microbiome and neuroinflammation in adolescent alcohol users. Early initiation of alcohol use, and binge drinking in particular, is considered one of the most important risk factors in the later development of alcohol and substance use disorders, but the neurobiological changes that may account for this increased vulnerability are not well understood. Alcohol-induced microbiome alterations at an early age may lead to neurometabolite alterations and in-turn affect the escalation of alcohol use. Consequently, the microbiome may be a novel area of research that may aid in adolescent AUD prevention and intervention efforts by providing new targets for treatment, and/or diagnostic and therapeutic response biomarkers. The present study therefore aims to investigate associations between the microbiota and neuroinflammation in the context of adolescent binge drinking. Specifically, the study will rely on analysis of oral microbiota 16S rRNA sequencing and magnetic resonance spectroscopy (MRS) data, in order to determine if binge drinking adolescents have higher levels of pro-inflammatory microbes and MRS markers of neuroinflammation. Together these data, regardless of if they are line with our hypotheses, will provide a foundation for neuroscience and microbiome informed targets and strategies for prevention and intervention efforts for problematic alcohol use. The research proposed in this F31 application will serve as essential hands-on training to promote Brittney's career development in adolescent alcohol use disorder neurobiology, neuroimaging, and the microbiome, and it will result in data on which to build an NIH F32 application.

项目总结/摘要 拟议的应用程序响应了更好地了解微生物组的需要， 青少年酒精使用者的神经炎症早期开始饮酒，特别是狂饮， 被认为是酒精和物质使用后期发展中最重要的风险因素之一 疾病，但可能导致这种脆弱性增加的神经生物学变化并不清楚。 明白早期酒精诱导的微生物组改变可能导致神经代谢物改变， 反过来影响酒精使用的升级。因此，微生物组可能是一个新的研究领域， 通过提供新的治疗目标，可以帮助青少年AUD预防和干预工作，和/或 诊断和治疗反应生物标志物。因此，本研究的目的是调查协会 在青少年酗酒的背景下，微生物群和神经炎症之间的联系。具体而言，研究 将依赖于对口腔微生物群16 S rRNA测序和磁共振光谱（MRS）数据的分析， 为了确定酗酒的青少年是否有更高水平的促炎微生物和MRS， 神经炎症的标志物不管这些数据是否符合我们的假设， 为神经科学和微生物组提供基础，为预防和 对有问题的酒精使用进行干预。F31申请中提出的研究将作为 基本的实践培训，以促进布兰妮的职业发展，在青少年酒精使用障碍 神经生物学，神经成像和微生物组，它将产生建立NIH F32的数据 应用程序.