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Discovery of a pigment produced by Streptococcus pyogenes

发现化脓性链球菌产生的色素

基本信息

批准号：
10680293
负责人：
MICHAEL J FEDERLE
金额：
$ 19.35万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10680293
关键词：
Acetyltransferase Acute Air Anabolism Antibiotic Therapy Bacteria Carotenoids Cell Density Cells Chemicals Chromatography Circulation Clinical Color Enzymes Event Exposure to Exudate Gene Family Genes Genetic Screening Growth Hand Human Hyperpigmentation Immune Infection Inflammatory Laboratories Ligase Lipids Literature Macrophage Methods Mutagenesis Nasopharynx Neutrophil Infiltration Nuclear Magnetic Resonance Operon Oranges Organism Oxidative Stress Oxygen Pathogenesis Pathway interactions Pharyngeal structure Phenotype Pigments Polyenes Production Property Pseudomonas aeruginosa Reactive Oxygen Species Recurrence Regulation Respiratory Burst Serotyping Skin Skin colonization Staphylococcus aureus Streptococcus Group B Streptococcus pyogenes Stress Structure Testing Tonsil Vaccines Virulence Virulence Factors Work antimicrobial bacterial fitness cell killing cytotoxic dehydrogenation environmental stressor fitness glycosylation glycosyltransferase human pathogen microorganism mutant new therapeutic target pathogen pathogenic bacteria stressor

项目摘要

Discovery of a pigment produced by Streptococcus pyogenes Abstract This proposal seeks to advance a new observation that the human pathogen Streptococcus pyogenes (Group A Streptococcus, GAS) produces a pigment that has gone unreported. We hypothesize the pigment protects GAS from host-derived and/or environmental stressors (such as reactive oxygen species) and likely enhances its fitness and potentially augments its virulence. For 80 years, no reference in the literature exists of a pigment being produced by this important organism, and prior to that, only two citations allude to such a factor. We suspect this property has been overlooked because its presence only becomes apparent in the laboratory after culturing in a chemically defined medium, grown to high cell densities, and after exposure to oxygen. Understanding if and how the production of a pigment could boost the fitness of GAS could provide a new target that therapeutics or vaccines could take aim upon. The objectives of this proposal are to elucidate the composition and structure of the GAS pigment, to identify genes associated with its production and regulation, and to characterize its ability to protect GAS from stressors and immune cell killing while exploring any cytotoxic properties.

化脓性链球菌产生色素的发现摘要这项提案旨在推进一项新的观察，即人类病原体化脓性链球菌（组一种链球菌（GAS）产生一种色素，这种色素尚未报道。我们假设色素可以保护来自宿主来源和/或环境应激源（如活性氧）的GAS，并可能增强它的适应性和潜在的增强其毒性。80年来，文献中没有提到一种颜料在此之前，只有两个引用提到了这样一个因素。我们我怀疑这种性质被忽视了，因为它的存在只有在实验室中才变得明显，在化学成分确定的培养基中培养，生长至高细胞密度，并且在暴露于氧气之后。了解色素的产生是否以及如何促进GAS的适应性可以提供一个新的治疗剂或疫苗可以瞄准目标。本提案的目的是阐明 GAS色素的组成和结构，以鉴定与其产生和调节相关的基因，并描述其保护GAS免受应激源和免疫细胞杀伤的能力，同时探索任何细胞毒性性质。