Tissue Culture/Molecular (TC/M) Core

项目总结(组织培养/分子) 17名视觉科学家申请继续资助(持有13项符合条件的NEI R01资助)和 他们的人员将支持三个资源/服务核心和一个行政核心 合并后的眼科、视觉和解剖科学系(原眼科 和解剖学/细胞生物学系)。组织培养/分子(TC/M)核心将是 由Jena Steinle博士指导，Jena Steinle博士是R01资助的研究员，在组织方面拥有重要的专业知识 培养和分子生物学技术。与私人侦探和一名研究助理一起， 韦恩州立大学及其附属机构进行视觉研究的环境和能力 将通过优先安排NEI R01资助的调查人员的工作来加强机构， 他们的教职员工和学生(研究生和研究员)在组织培养和 分子生物学。TC/M核心将为视觉科学家(优先资助NEI R01)提供 确保工作质量和成本效益的设施和技术援助 已执行。该核心将维护和繁殖眼部和其他类型的细胞，并提供 基因分型、逆转录聚合酶链式反应(RT)和 定量聚合酶链式反应(Q)-PCR、基因沉默-siRNA、shRNA、CRISPR/Cas9、 套式聚合酶链式反应和依从性试验。它还将就以下问题提供咨询/援助 重组(R)AAV病毒载体的设计和构建以及对PI、学生或 进行体外功能评估的工作人员。总体而言，TC/M核心将协助NEI R01资助 研究人员及其教职员工/学生需要使用组织培养和 分子生物学技术；利用组织培养促进视觉科学家之间的合作 和分子生物技术；并通过以下方式将新的研究人员带入视觉研究 在组织培养和分子技术方面提供帮助，以扩大他们的研究 全神贯注。

Project Summary (Tissue Culture/Molecular) Continuing funds are requested by 17 vision scientists (holding 13 eligible NEI R01 grants) and their personnel to support three resource/service cores and an administrative core in the newly merged Department of Ophthalmology, Visual and Anatomical Sciences (former Ophthalmology and Anatomy/Cell Biology Departments). The Tissue Culture/Molecular (TC/M) core will be directed by Dr. Jena Steinle, an R01-funded investigator with significant expertise in tissue culture and molecular biology techniques. Together with the PI, and a research assistant, the environment and capability to conduct vision research at Wayne State University and affiliated institutions will be enhanced through prioritization of the work of NEI R01 funded investigators, their staff and students (graduate and fellows) in numerous aspects of both tissue culture and molecular biology. The TC/M core will provide vision scientists (priority for NEI R01 funded) with facilities and technical assistance that will assure quality and cost effectiveness of the work performed. This core will maintain and propagate ocular and other cell types, as well as provide investigators with genotyping, reverse transcription-polymerase chain reaction (RT) and quantitative polymerase chain reaction (q)-PCR, gene silencing- siRNA, shRNA, CRISPR/Cas9, nested PCR and adherence assays. It will also provide for consultation/assistance on recombinant (r) AAV virus vector design and construction and assistance to the PI, students or staff for in vitro functional assessments. Overall, the TC/M core will assist NEI R01-funded investigators and their staff/students in studies that require the use of tissue culture and molecular biology techniques; foster collaboration between vision scientists using tissue culture and molecular biological techniques; and bring new investigators into vision research by providing assistance in tissue culture and molecular techniques that can expand their research focus.