Flow Cytometry

流式细胞仪

• 批准号: 10686131
• 负责人: Aniruddha J. Deshpande
• 金额: $ 21.23万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10686131
• 关键词: Acceleration; Antibodies; Cancer Center; Cancer Center Support Grant; Cell Separation; Cells; Collaborations; Color; Communication; Computer software; Contract Services; Core Facility; Cytometry; Data Analyses; Development; Doctor of Philosophy; Emerging Technologies; Employee; Equipment; Evaluation; Experimental Designs; Faculty; Feedback; Flow Cytometry; Flow Cytometry Shared Resource; Funding; Grant; Image; Image Cytometry; Immune; Immunophenotyping; Institution; Lasers; Leadership; Licensing; Maintenance; Malignant Neoplasms; Manuscripts; Microfluidics; Monitor; NCI-Designated Cancer Center; Patients; Phenotype; Population; Preparation; Protocols documentation; Publications; Research; Research Personnel; Resource Sharing; Science; Scientist; Services; Site; Solid Neoplasm; Sorting; Speed; Stains; Surveys; Suspensions; Technology; Training; Work; cost effectiveness; experience; experimental study; instrument; instrumentation; investigator training; meetings; member; programs; repaired; skills; technology platform; tumor

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource offers Cancer Center members multi-parametric evaluation and high- speed enrichment of phenotypically distinct cell populations. The Core provides full-service high-speed cell sorting performed by expert operators, utilizing two BD Aria instruments in biosafety enclosures. For analytical cytometry, investigators have a choice of either full-service analysis by Core staff or receiving training and then independently using one of six analytical flow cytometers. Those analytical cytometers, available 24/7 to trained users, include two BD Fortessas, the newer of which is a 5-laser, 18-color instrument, and an ImageStreamX MkII imaging flow cytometer with four lasers and 12 imaging channels. Many of the Core's cytometers have autoloaders, enabling larger-scale experiments. Analysis software is available in the Core or via the Institutional site license. This broadly used Core is managed by the CC and receives support from the Institute for instrumentation purchase and maintenance. In the last funding period, three major technology platforms were acquired, and imaging flow cytometry was launched as a new service. The Core Director, Mr. Altman, and staff have combined experience of more than 30 years in NCI-designated Cancer Center Flow Cytometry Cores, and they provide comprehensive technical guidance at any stage of a project, from experimental design through manuscript preparation. The Flow Cytometry Core is broadly used, as in the last 5 years, it was used by 46 Cancer Center labs representing all three programs, and the Core contributed to at least 75 cancer-related publications.

摘要-流式细胞术共享资源