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Genome Engineering Core

基因组工程核心

基本信息

批准号：
10704276
负责人：
MEGAN Anne COOPER
金额：
$ 22.9万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10704276
关键词：
Autoimmune Diseases Award CRISPR/Cas technology Cell Line Cell model Cells Clinical Investigator Clustered Regularly Interspaced Short Palindromic Repeats Communities Consultations Data Analyses Development Disease Disease model Education Education and Outreach Embryo Engineering Equipment Funding Gene Targeting Generations Genetic Genetic Engineering Genetic Services Genetically Engineered Mouse Genome Genome engineering Genomics Goals Grant Individual Inflammatory Infrastructure Investigation Knockout Mice Laboratories Leadership Mission Modeling Mus Mutation Patients Pre-Clinical Model Production Publications Reagent Research Personnel Research Support Resources Rheumatism Services Techniques Technology Testing Transgenic Organisms Translational Research Universities Washington Work career community center cost effective disease phenotype education resources experience genetic technology genetically modified cells genome editing genome sequencing genomic data induced pluripotent stem cell innovation meetings member model organism mouse model novel pre-clinical research and development skills stem cell model stem cells success targeted treatment tool transcriptome sequencing translational genetics translational scientist

项目摘要

GENOME ENGINEERING CORE - ABSTRACT The goal of the Genome Engineering Core (GEC) of the Washington University Rheumatic Diseases Research Resource-based Center (WU-RDRRC) is to provide access to innovative genomic technologies to build tools that will facilitate pre-clinical modeling and functional testing for the investigation of inflammatory and autoimmune disease. Genomic investigation of the basis of rheumatic disease has become widespread. However, the translation of genetic findings can be challenging, requiring development of models to interrogate basic questions of cellular and disease phenotype and function due to identified genetic changes. This requires development of validated models through innovative genome engineering technologies. These technologies, such as development of genetically-engineered mice and CRISPR/Cas9 editing of induced pluripotent stem cells (iPSCs) and mouse embryos require significant experience and highly-specialized equipment not available within most laboratories. The GEC provides WU-RDRRC investigators with access to the latest in genomic engineering techniques by leveraging services from the Transgenic and Knockout Mouse Facility (TKMF) and the Genome Engineering & Stem Cell Center at McDonnell Genome Institute (GESC), with education and leadership to facilitate projects. We propose to continue these services and innovate the GEC through following three Specific Aims: 1. Sustain, develop, and introduce new state-of-the-art genetics services to WU-RDRRC members. 2. Assist investigators in the generation of genetically engineered mouse models by partnering with the Transgenic and Knockout Mouse Facility (TKMF). 3. Assist investigators in the generation of reagents for creation of genetically-engineered cell lines, iPSCs, and mouse models by leveraging the Genome Engineering & Stem Cell Center at McDonnell Genome Institute (GESC).

基因组工程核心-摘要