Virus Egress Pathways

病毒出口途径

• 批准号: 10706182
• 负责人: Nihal Altan-Bonnet
• 金额: $ 248.47万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10706182
• 关键词: 3-Dimensional; Animals; Antigen Presentation; Astrovirus; Cell Culture Techniques; Cell physiology; Cells; Clinical; Coronavirus; Decontamination; Defect; Endothelium; Enteral; Epithelial; Homeostasis; Human; Immune; Immune response; Immunobiology; In Vitro; Lead; Lipids; Lung; Lysosomes; Manuscripts; Modeling; Norovirus; Pathway interactions; Population; Process; Public Health; Research; Rotavirus; Route; Saliva; Salivary; Salivary Glands; Site; Vesicle; Viral; Virulence; Virus; Water; adaptive immune response; extracellular vesicles; in vivo; novel; particle; transmission process; viral transmission

Coronavirus Egress Research- lysosomal release of viruses: We have identified the novel egress pathway exploited by coronaviruses. We have found that these viruses use lysosomes to be transported out of the cell. In the process of using lysosomes these viruses disrupt lysosomal function. We have found that this leads to profound defects in cell physiology including antigen presentation and may lead to the observed clinical abnormalities. Other positive strand virus egress research: We have an entirely novel mode of viral transmission, whereby viruses are transmitted en masse or en bloc , inside extracellular vesicles. We are studying the impact of this form of transmission on the host innate and adaptive immune response. We also discovered a novel mode of transmission and a novel site of replication for enteric viruses including norovirus, rotavirus, astrovirus and others. We have found that these viruses replicate robustly in the salivary glands and transmit to others via saliva. This finding is novel and has significant public health implications. The manuscript detailing this discovery is under review.

冠状病毒出口研究--病毒的溶酶体释放： 我们已经发现了冠状病毒利用的新的出口途径。我们已经发现，这些病毒使用溶酶体运出细胞。在使用溶酶体的过程中，这些病毒扰乱了溶酶体的功能。我们发现，这会导致包括抗原提呈在内的细胞生理学方面的严重缺陷，并可能导致观察到的临床异常。 其他正链病毒出口研究：我们有一种全新的病毒传播模式，病毒在细胞外小泡内集体或整体传播。我们正在研究这种传播形式对宿主先天和获得性免疫反应的影响。 我们还发现了肠道病毒包括诺如病毒、轮状病毒、星状病毒等的一种新的传播方式和新的复制位点。我们发现，这些病毒在唾液腺中复制旺盛，并通过唾液传播给其他人。这一发现是新颖的，并具有重大的公共卫生影响。详细描述这一发现的手稿正在审查中。