Coupled Transfers of Electrons and Protons

电子和质子的耦合转移

• 批准号: 10798557
• 负责人: JAMES M MAYER
• 金额: $ 3.23万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798557
• 关键词: Acceleration; Biochemical; Biological Models; Chromatography; Column Chromatography; Complex; Coupled; Electron Transport; Energy Transfer; Equipment; Fractionation; Funding; Kinetics; Laboratories; Modeling; Oxidation-Reduction; Pathway interactions; Phase; Preparation; Process; Protons; Pump; Reporting; Research; System; Techniques; Time; chemical reaction; chemical synthesis; design; detector; improved; pressure

Project summary/abstract Supplemental funding is requested for the purchase of an automated flash chromatography system. This automated system will greatly facilitate and accelerate the chemical synthesis of model compounds to develop fundamental principles of proton-coupled electron transfer (PCET) and the recently discovered proton-coupled energy transfer (PCEnT) process (reported summer 2022). The synthesis of new pure compounds for study is often the slow step in this research. The requested system is comprised of high-pressure pumps, pre-packed columns of various sizes and stationary phases, and a photodiode array detector for fractionation. This system fully automates and improves the traditional time-intensive and labor-intensive column chromatography for separation of the products of chemical reactions. This system will enable the rapid and efficient purification of both the desired compounds for study and the synthetic intermediates along the pathway to the final products. Therefore, the automated flash chromatography system will accelerate the planned research and allow extensions to wider groups of compounds and to new model systems. More ready availability of compounds to study are particularly important in the growing collaborative efforts, in which our laboratory has been the generator and synthesizer of new molecules. In particular, the team’s discovery of PCEnT brings new challenges to synthesize molecules that will proceed by this pathway or by PCET.

项目摘要/摘要 要求提供补充资金，用于购买自动闪光层析仪 系统。这一自动化系统将极大地促进和加速化学合成 发展质子耦合电子转移(PCET)基本原理的模型化合物 以及最近发现的质子耦合能量转移(PCEnT)过程(报道于夏季 2022年)。合成用于研究的新的纯化合物往往是这项研究中缓慢的一步。 所要求的系统由高压泵、各种尺寸的预填充柱组成。 和固定相，以及用于分离的光电二极管阵列检测器。该系统充分利用了 自动化并改进传统的时间密集型和劳动密集型栏目 用于分离化学反应产物的层析法。该系统将使 快速高效地提纯所需研究用化合物和合成化合物 中间体沿着通向最终产品的路径。因此，自动闪光灯 层析系统将加快计划中的研究，并允许扩展到更广泛的范围 化合物群和新的模型系统。更容易获得可供研究的化合物 在不断增长的合作努力中尤为重要，我们的实验室一直在 新分子的生成器和合成器。特别是，该团队对PCEnT的发现 为合成将通过这一途径或通过PCET进行的分子带来了新的挑战。