Coupled Transfers of Electrons and Protons

电子和质子的耦合转移

• 批准号: 10798557
• 负责人: JAMES M MAYER
• 金额: $ 3.23万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798557
• 关键词: Acceleration; Biochemical; Biological Models; Chromatography; Column Chromatography; Complex; Coupled; Electron Transport; Energy Transfer; Equipment; Fractionation; Funding; Kinetics; Laboratories; Modeling; Oxidation-Reduction; Pathway interactions; Phase; Preparation; Process; Protons; Pump; Reporting; Research; System; Techniques; Time; chemical reaction; chemical synthesis; design; detector; improved; pressure

Project summary/abstract Supplemental funding is requested for the purchase of an automated flash chromatography system. This automated system will greatly facilitate and accelerate the chemical synthesis of model compounds to develop fundamental principles of proton-coupled electron transfer (PCET) and the recently discovered proton-coupled energy transfer (PCEnT) process (reported summer 2022). The synthesis of new pure compounds for study is often the slow step in this research. The requested system is comprised of high-pressure pumps, pre-packed columns of various sizes and stationary phases, and a photodiode array detector for fractionation. This system fully automates and improves the traditional time-intensive and labor-intensive column chromatography for separation of the products of chemical reactions. This system will enable the rapid and efficient purification of both the desired compounds for study and the synthetic intermediates along the pathway to the final products. Therefore, the automated flash chromatography system will accelerate the planned research and allow extensions to wider groups of compounds and to new model systems. More ready availability of compounds to study are particularly important in the growing collaborative efforts, in which our laboratory has been the generator and synthesizer of new molecules. In particular, the team’s discovery of PCEnT brings new challenges to synthesize molecules that will proceed by this pathway or by PCET.

项目概要/摘要 请求提供补充资金，用于购买一台自动快速色谱仪 系统这一自动化系统将极大地促进和加速化学合成 模型化合物，以开发质子耦合电子转移（PCET）的基本原理 和最近发现的质子耦合能量转移（PCEnT）过程（夏季报道） 2022年）。合成新的纯化合物用于研究往往是这一研究的缓慢步骤。 所要求的系统由高压泵、各种尺寸的预填充柱组成 和固定相，以及用于分馏的光电二极管阵列检测器。该系统完全 自动化和改进传统的时间密集型和劳动密集型柱 用于分离化学反应产物的色谱法。该系统将使 快速和有效地纯化用于研究的所需化合物和合成的化合物。 沿着通向最终产物的途径的中间体。因此，自动闪光灯 色谱系统将加速计划中的研究，并允许扩展到更广泛的 化合物组和新的模型系统。更容易获得化合物进行研究 在不断增长的合作努力中尤为重要，我们的实验室一直在 新分子的发生器和合成器。特别是，研究小组发现的PCEnT 为合成通过该途径或通过PCET进行的分子带来了新的挑战。