High-Precision Non-Contact Plasmon-Induced Intracellular Delivery

高精度非接触式等离激元诱导细胞内递送

• 批准号: 10813943
• 负责人: Naihao Chiang
• 金额: $ 4.94万
• 依托单位: UNIVERSITY OF HOUSTON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-22 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10813943
• 关键词: Address; Award; Basic Science; Biochemical; Biosensing Techniques; Cell Culture Techniques; Cell Nucleus; Cell Survival; Cell membrane; Cell surface; Cells; Cellular biology; Chemicals; Chemistry; Clinical; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Computers and Advanced Instrumentation; Custom; Data; Dedications; Development Plans; Educational workshop; Electromagnetic Fields; Electron Microscopy; Electroporation; Environment; Feedback; Foundations; Future; Gene Delivery; Genes; Human; Image; Immune system; Immunotherapy; Ion Channel; Ions; Label; Laboratories; Membrane; Membrane Proteins; Mentorship; Methods; Microfluidic Microchips; Microfluidics; Microscope; Modality; Occupations; Optics; Outcome; Parents; Pathway interactions; Penetration; Performance; Persons; Phase; Physicians; Physics; Population; Postbaccalaureate; Process; Protocols documentation; Raman Spectrum Analysis; Regenerative Medicine; Research; Research Activity; Research Personnel; Resolution; Route; Scanning; Scientist; Site; Spectrum Analysis; Stains; Sterilization; Surface Plasmon Resonance; System; T-Lymphocyte; Techniques; Technology; Testing; Time; Training; Transfection; Transferable Skills; Update; career; career development; cell fixing; cellular imaging; chimeric antigen receptor T cells; delivery vehicle; design; embryonic stem cell; gene therapy; genome editing; imaging capabilities; imaging platform; improved; innovation; insight; interest; nano; nanoGold; nanoscale; parallelization; plasmonics; single molecule; skills; stem; stem cell biology; stem cells; symposium; targeted delivery; targeted nucleases; tool; translational medicine

ABSTRACT This research supplement aims to enhance the career development a post-baccalaureate researcher from underrepresented backgrounds in the PI’s group. At the same time, the supplementary research activity is designed to provide complimentary data to the parental research activities, and therefore, to build a foundation on common and translatable biomedical laboratory skills for the trainee. Specifically, we will focus on basic techniques such as sterilization, cell culture, cell fixing and staining, and optical and electron microscopies with more advanced instrumentation that are relevant to the day-to-day research activities in cell biology. These highly transferable skills will benefit the trainee’s career as a future physician, scientist, or both. The career development plan will include a close mentorship from the PI, in-person training on Responsible Conduct in Research (RCR), and networking from research collaborations and regional and national seminars, workshops, and conferences. Ultimately, the research and soft skills developed in this proposed supplement will be a cornerstone for the trainee’s future career in biomedical-related occupations and therefore contribute to a more diverse and inclusive biomedical community.

摘要 本研究补充旨在提高职业发展后学士学位的研究人员从 私家侦探小组中代表性不足的背景同时，补充研究活动是 旨在为父母的研究活动提供补充数据，因此， 为受训者提供通用和可翻译的生物医学实验室技能。具体而言，我们将重点关注基本 技术，如灭菌，细胞培养，细胞固定和染色，以及光学和电子显微镜， 与细胞生物学的日常研究活动相关的更先进的仪器。这些高度 可转移的技能将有利于受训者未来成为医生、科学家或两者兼而有之。职业发展 计划将包括PI的密切指导，关于研究中负责任行为（RCR）的面对面培训， 通过研究合作以及区域和国家研讨会、讲习班和会议建立网络。 最终，在这一拟议补充中开发的研究和软技能将成为 培训生未来在生物医学相关职业的职业生涯，因此有助于更多样化和包容性 生物医学社区

项目成果

Exploring the Bottom-Up Growth of Anisotropic Gold Nanoparticles from Substrate-Bound Seeds in Microfluidic Reactors.

• DOI: 10.1021/acsanm.3c00440
• 发表时间: 2023-04-28
• 期刊: ACS APPLIED NANO MATERIALS
• 影响因子: 5.9
• 作者: Vinnacombe-Willson, Gail A.;Lee, Joy K.;Chiang, Naihao;Scarabelli, Leonardo;Yue, Shouzheng;Foley, Ruth;Frost, Isaura;Weiss, Paul S.;Jonas, Steven J.
• 通讯作者: Jonas, Steven J.

Extraction of Hidden Science from Nanoscale Images.

• DOI: 10.1021/acs.jpcc.1c08712
• 发表时间: 2022-01-13
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY C
• 影响因子: 3.7
• 作者: Barr, Kristopher K.;Chiang, Naihao;Bertozzi, Andrea L.;Gilles, Jerome;Osher, Stanley J.;Weiss, Paul S.
• 通讯作者: Weiss, Paul S.