Enhancing Performance of Antimicrobial Susceptibility Testing (AST) in Support of Vet-LIRN Activities

提高抗菌药物敏感性测试 (AST) 的性能以支持 Vet-LIRN 活动

• 批准号: 10828498
• 负责人: Kelli Maddock
• 金额: $ 8.46万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10828498
• 关键词: Enhancing; Performance; Antimicrobial; Susceptibility; Testing

Project Summary/Abstract: This comparison study is intended to compare Sensititre™ Vet AST COMPGP1F/COMPGN1F plates to bioMérieux Vitek® AST-GP81/AST-GN96 cards for performance of antimicrobial susceptibility testing (AST) results. This method comparison will be performed on common bacterial companion animal pathogens such as Escherichia coli, Enterococcus species, Staphylococcus pseundintermedius, Pseudomonas aeruginosa, and other Enterobacterales isolates. These bacterial pathogens may be shared between companion animals and humans; therefore, antimicrobial resistance (AMR) of these isolates is a critical concern. AST results on companion animals are significant for prediction of treatment outcome and are valuable comparisons to AMR trends occurring in humans. Although widely used in veterinary microbiology, AST plates manufactured by the currently dominating vendor, Sensititre™, are lacking the necessary range of antimicrobials to adequately detect common resistance mechanisms which would allow for appropriate comparison to AMR trends occurring in humans. The bioMérieux Vitek is commonly used in human microbiology laboratories; AST cards designed for this system incorporate ranges of antimicrobial concentrations of clinical and epidemiological interest for key indicators of antimicrobial resistance. Although the manufacturer has products designed for veterinary use, few laboratories in the United States use the bioMérieux Vitek instrument for AST. To date, there are no published studies comparing Sensititre™ AST to bioMérieux Vitek. Findings of this methods comparison will be published, which will provide objective data that veterinary diagnostic laboratories can use to determine which method is most appropriate for their laboratories. This comparison study will enhance AMR surveillance capabilities of the NDSU VDL and will directly support Vet-LIRN initiatives of protecting public health. This study may identify a reliable and affordable second vendor for AST in veterinary microbiology, which also ensures that all vendors continue offering useful veterinary products and encourages routine improvements of products offered to veterinary diagnostic laboratories.

项目概要/摘要： 本比较研究旨在比较Sensititre™ Vet AST COMPGP 1F/COMPGN 1F 将平板与bioMérieux Vitek® AST-GP 81/AST-GN 96药敏卡进行比较，以确定抗菌性能 药敏试验（AST）结果。该方法比较将在常见的 细菌性伴侣动物病原体如大肠杆菌，肠球菌属， 假中间葡萄球菌、铜绿假单胞菌和其他肠球菌目 分离株这些细菌病原体可能在伴侣动物和人类之间共享; 因此，这些分离株的抗菌素耐药性（AMR）是一个关键问题。AST结果 伴侣动物对于预测治疗结果具有重要意义， 与人类发生的AMR趋势进行比较。虽然广泛用于兽医 微生物学方面，目前主要供应商Sensititre™生产的AST平板， 缺乏必要的抗菌剂范围以充分检测常见耐药性 这些机制允许与人类中发生的AMR趋势进行适当比较。 bioMérieux Vitek常用于人体微生物实验室; AST卡 为该系统设计的抗菌剂浓度范围包括临床和 流行病学兴趣的抗菌素耐药性的关键指标。虽然 制造商有专为兽医使用的产品，在美国很少有实验室使用 bioMérieux Vitek AST仪器。到目前为止，还没有发表的研究比较 Sensititre™ AST至bioMérieux Vitek。将公布这一方法比较的结果， 这将为兽医诊断实验室提供客观数据， 哪种方法最适合他们的实验室。这项比较研究将提高 NDSU VDL的AMR监测功能并将直接支持Vet-LIRN计划 保护公众健康。这项研究可以确定一个可靠和负担得起的第二供应商， AST在兽医微生物学，这也确保了所有供应商继续提供有用的 兽医产品，并鼓励对提供给兽医的产品进行常规改进 诊断实验室