ADRDL Diagnostic testing support for the FDA CVM VET-LIRN program

ADRDL 对 FDA CVM VET-LIRN 计划的诊断测试支持

• 批准号: 10828686
• 负责人: Muhammed Muyyarikkandy
• 金额: $ 7.5万
• 依托单位: SOUTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-10 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10828686
• 关键词: ADRDL; Diagnostic; testing; support; FDA

4/12/23 Project Summary The South Dakota Animal Disease Research and Diagnos�c Laboratory (SD ADRDL) will serve as a Whole Genome Sequencing (WGS) core laboratory for the Veterinary Laboratory Inves�ga�on and Response Network (Vet-LIRN). The main aim of this project is to perform WGS on isolates obtained from working with 5 partner source labs: North Carolina State University Center for Veterinary Medicine, Michigan State University Veterinary Diagnos�c Laboratory, Minnesota Veterinary Diagnos�c Laboratory, Iowa State University Veterinary Diagnos�c Laboratory, and Oklahoma Animal Disease Diagnos�c Laboratory. These partners will culture Salmonella enterica, Escherichia coli, Staphylococcus pseudintermedius, and other relevant organisms from veterinary matrices. Once sent to the SD ADRDL, iden�ty will be verified via MALDI-TOF before extrac�ng genomic DNA and performing WGS. Sequence will be uploaded to NCBI bioprojects designated by Vet-LIRN Program Office. Vet-LIRN has been tasked with developing AST and WGS assays that will allow for increased understanding of an�microbial resistance within veterinary samples. This knowledge will be used to develop evidence-based interven�ons that have demonstrated reduc�ons in resistance. 5U18FD006558 Page 1 of 1

4/12/23 项目摘要 南达科他州动物疾病研究和诊断实验室（SD ADRDL）将作为一个整体 基因组测序（WGS）兽医实验室的核心实验室调查和响应 网络（Vet-LIRN）。本项目的主要目的是对从工作中获得的分离株进行WGS 与5个合作伙伴源实验室：密歇根州北卡罗来纳州州立大学兽医中心 州立大学兽医诊断实验室，明尼苏达州兽医诊断实验室，爱荷华州 州立大学兽医诊断实验室和俄克拉荷马州动物疾病诊断实验室。 这些合作伙伴将培养肠道沙门氏菌，大肠杆菌，假中间葡萄球菌， 兽医基质中的其他相关微生物。一旦发送到SD ADRDL，身份将被验证艾德 在提取基因组DNA和进行WGS之前通过MALDI-TOF。序列将上传至NCBI 由Vet-LIRN计划办公室指定的生物项目。 Vet-LIRN的任务是开发AST和WGS检测， 了解兽医样品中的微生物耐药性。这些知识将用于 发展以证据为基础的干预措施，这些措施已经证明了耐药性的减少。 5 U18 FD 006558第1页，共1页