Core-007

摘要 芝加哥大学医学综合癌症中心（UCCCC）旨在改善预防， 通过我们的基础，临床，转化和人口研究活动检测和治疗癌症。 因此，人体生物标本的有效获取、储存、使用和分析至关重要 重要性，这些最先进设施的维护和扩建对未来的成功至关重要 我们的癌症项目。成立于2001年，人体组织资源中心（HTRC）是一个学院， 美国病理学家（CAP）认证的生物储存库。我们的使命是有效地采购、储存、分发， 和分析生物标本，从而为研究人员提供一个集中的基础设施，以优化 与涉及生物标本的研究有关的效率和成本。该设施占地约4，000平方米。英尺的 病理科内部的翻新空间，作为一个综合单位发挥作用。HTRC由 四个集成子核心：生物标本库、激光捕获显微切割、数字病理学和 组织学服务。目前，HTRC提供收集临床注释的人类 组织以及血液、血清、血浆和唾液、尿液和外周血单核细胞用于癌症相关研究。此外，该中心还为组织病理学、LCM、 组织微阵列制备和核酸提取。通过这种方式，我们提供了一个协调的，集中的， 以及用于获取、处理、分散和评估所有类型生物标本的专用程序。期间 在当前CCSG资助期间，HTRC经历了急剧的扩张。例如数目 生物库方案的数量从114（2013年）增加到253（2017年）。此外，我们还增加了 收集的生物样本类型，特别是关于生物流体（血浆、血清、淋巴细胞DNA和 外周血单核细胞分离）。因此，每年库存的标本数量从 18，682（CY2013）至31，091（CY2016）。同样，组织学服务显示， 2013年使用88，338个单位进行组织切片和染色，2016年使用95，713个单位完成染色。 HTRC也取得了一些重要进展。其中包括收购2 Cryostar NX50 HD Low Profile低温恒温器、Leica LMD 6500 LCM显微镜、Autogen FlexStar DNA 分离机和Leica Bond RX自动化IHC染色机。重要的是，HTRC已迁移到 LabVantage LIMS指导我们的生物库流程。LabVantage是一个基于Web的密码保护示例 和信息管理系统。它包含机构审查委员会（IRB）和知情同意信息， 基本的患者人口统计数据和详细的生物样本信息。LabVantage自动分配唯一标识符 （条形码）并提供实时样品跟踪数据。未来的主要计划集中在增加我们的 液体处理和生物样本储存的自动化。随着我们的不断发展， 自动化将提高我们的效率，减少人为错误的风险，并简化我们的许多流程。

ABSTRACT The University of Chicago Medicine Comprehensive Cancer Center (UCCCC) aims to improve the prevention, detection, and treatment of cancer through our basic, clinical, translational, and population research activities. Therefore, the effective procurement, storage, use, and analysis of human biospecimens are of critical importance, and the maintenance and expansion of these state-of-the-art facilities is vital to the future success of our cancer programs. Established in 2001, the Human Tissue Resource Center (HTRC) is a College of American Pathologists (CAP) accredited biorepository. Our mission is to effectively procure, store, distribute, and analyze biospecimens, thereby providing investigators with a centralized infrastructure to optimize the efficiency and costs related to research involving biospecimens. The Facility occupies ~4,000 sq. ft. of renovated space within the Pathology Department and functions as an integrated unit. The HTRC is comprised of four integrated Subcores: Biospecimen Bank, Laser Capture Microdissection, Digital Pathology, and Histology Services. Currently, the HTRC provides services for the collection of clinically-annotated human tissues, as well as blood, serum, plasma, and saliva, urine, and peripheral blood mononuclear cells for cancerrelated research. In addition, the Core provides collaborative support and services for histopathology, LCM, tissue microarray preparation, and nucleic acid extraction. In this way, we provide a coordinated, centralized, and dedicated program for procuring, processing, dispersing, and assessing all types of biospecimens. During the current CCSG funding period, the HTRC has undergone a dramatic expansion. For example, the number of biobanking protocols has increased from 114 (CY2013) to 253 (CY2017). In addition, we have increased the types of biospecimens collected, particularly with respect to biofluids (plasma, serum, lymphocyte DNA, and peripheral blood mononuclear cell isolation). As a result, the number of annually banked specimens rose from 18,682 (CY2013) to 31,091 (CY2016). Similarly, histology services show a continued increase in requests for tissue sections and staining with 88,338 units performed in CY2013 and 95,713 units completed in CY2016. The HTRC has also undergone a number of important advances. These have included the acquisition of 2 Cryostar NX50 HD Low Profile cryostats, a Leica LMD 6500 LCM microscope, an Autogen FlexStar DNA isolation machine, and a Leica Bond RX automated IHC Stainer. Importantly, the HTRC has migrated to LabVantage LIMS to guide our biobanking process. LabVantage is a web-based, password-protected sample and information management system. It contains Institutional Review Board (IRB) and consent information, basic patient demographics, and detailed biospecimen information. LabVantage auto-assigns unique identifiers (barcodes) and provides real-time sample tracking data. Major future plans are focused on increasing our automation of liquid handling and biospecimen storage. As we continue to grow, the implementation of automation will increase our efficiency, decrease risks of human error, and streamline many of our processes.