RFA-GH-20-005, Conducting Integrated Infectious Disease and Public Health Research in Peru

RFA-GH-20-005，在秘鲁进行综合传染病和公共卫生研究

• 批准号: 10842492
• 负责人: Margaret N Kosek
• 金额: $ 10万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2025-09-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10842492
• 关键词: RFA; GH; 20; 005; Conducting

PROJECT SUMMARY: The proposed application aims to overcome diagnostic barriers in the etiologic diagnosis of endemic and epidemic causes of acute febrile illness (AFI) by moving efficient state of the art diagnostics to an area of high endemicity. The long-term goal of this research is to understand the etiology of AFI across diverse contexts in South America and contribute to global strategies that improve etiology-specific disease estimates to inform public health practice and policy. The central hypothesis is that the technology transfer and implementation of improved diagnostics will enhance regional diagnostic capacity, epidemic responsiveness, and translation of research findings to practice maximizing the public health impact of research findings. The rationale for the research is that the documented feasibility of this approach will enable a full and appropriate evaluation for uptake by regional and national stakeholders. The goals of the research will be achieved through the execution of three specific aims: 1) Establish stable year round acute febrile illness surveillance using the TaqMan array card, which simultaneously detects over 30 pathogens of AFI across three epidemiologic contexts in Loreto, Peru; urban, riverine with extensive sylvatic exposure, and agricultural riverine context along the three country border (Peru, Brazil, and Colombia) in order to capture the diversity of pathogens causing AFI in Peru over a four year period; 2) Integrate this diagnostic platform into the regional outbreak response to determine the etiology of epidemics of acute febrile illness and validating improved storage and transport procedures; and 3) Develop and evaluate novel serodiagnostic chip to enable single platform serologic assessment and downstream point of care tests and focused diagnostics for pathogens of high prevalence and importance. The global control of emerging infectious diseases will be improved only as the areas that are not reached and included effectively in surveillance shrink and the proposed project attempts to implement a strategy to make all populations reachable to the improved diagnostics. The complexity of current diagnostic strategies is a critical barrier to the timely regional ascertainment of the etiology of most cases of acute febrile illnesses and this has important regional and global public health implications as undiagnosed AFI allows for the widespread dissemination of emerging diseases as effective disease control measures are based on accurate etiologic assessment. The plan is innovative because of the meaningful integration of highly select local researchers, educators, trainees, and policymakers. The implementation of the research strategy will be significant because it is expected to contribute to the accuracy of etiologic assessment of endemic and epidemic etiologies of AFI in an area of documented diversity of vector-borne and zoonotic agents and serve as a model for enabling local diagnostic and reporting capacity in South America.

项目总结： 拟议的应用旨在克服地方性和地方性疾病病因诊断方面的诊断障碍。 通过将高效的最先进的诊断技术转移到一个高风险的地区，导致急性发热性疾病(AFI)的流行原因 地方性。这项研究的长期目标是在不同的背景下了解AFI的病因 并为改善特定病因学疾病估计的全球战略做出贡献，以告知 公共卫生实践和政策。中心假设是，技术转让和实施 改进的诊断将增强区域诊断能力、流行病反应能力和翻译 研究成果以实践最大限度地发挥研究成果对公众健康的影响。该计划的基本原理 研究表明，这种方法的记录可行性将使全面和适当的评估 区域和国家利益攸关方的理解。研究的目标将通过执行来实现 三个具体目标：1)使用TaqMan阵列建立全年稳定的急性发热病监测 CARD在洛雷托的三个流行病学背景下同时检测30多种AFI病原体， 秘鲁；三个国家沿线的城市、河流和农业河流环境 边境(秘鲁、巴西和哥伦比亚)，以捕捉在秘鲁引起AFI的病原体的多样性 四年期间；2)将这一诊断平台纳入区域疫情应对，以确定 急性发热性疾病流行的病原学和验证改进的储存和运输程序；以及3) 开发和评估新的血清诊断芯片，以实现单平台血清学评估和下游 对高流行率和高重要性的病原体进行护理点测试和重点诊断。全球控制 只有当新发传染病没有得到有效覆盖和纳入时，新发传染病的情况才会得到改善 在监测方面缩减，拟议的项目试图实施一项战略，使所有人口 可获得改进的诊断。当前诊断策略的复杂性是阻碍 及时确定大多数急性发热性疾病的病因，这具有重要的意义 未诊断的AFI对区域和全球公共卫生的影响允许广泛传播 新出现的疾病作为有效的疾病控制措施，是建立在准确的病原学评估基础上的。这个计划 具有创新性，因为高度精选的当地研究人员、教育工作者、受训人员和 政策制定者。研究战略的实施将具有重大意义，因为预计它将有助于 对有文件记载的地区AFI流行病原学评估的准确性 病媒和人畜共患病病原体的多样性，并作为促进当地诊断和报告的模式 南美的运力。