Detection of Emergent Mechanical Properties of Biologically Complex Cellular States

生物复杂细胞状态的紧急机械特性的检测

• 批准号: 10838854
• 负责人: Mark A LaBarge
• 金额: $ 4.81万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10838854
• 关键词: Age; Applications Grants; Appointment; BRCA1 gene; BRCA2 gene; Biomedical Engineering; Breast Cancer Risk Factor; Breast Epithelial Cells; Chronology; Communities; Complex; Detection; Development Plans; Devices; Disparity; Dose; Family; First Generation College Students; Genetic; Human; Laboratory Research; MCF10A cells; Machine Learning; Measures; Mechanics; Memory; Mentors; Modeling; Molecular; Non-Malignant; PALB2 gene; Pathway interactions; Predisposition; Recording of previous events; Role; Testing; Variant; Woman; career development; design; doctoral student; experimental study; graduate school; in silico; innovation; malignant breast neoplasm; mechanical properties; older women; parent grant; pre-doctoral; programs; prototype; young woman

Abstract This is a Diversity Supplement to R01EB024989 for the career development of Taylor Thomsen, a first- generation college student who is entering her 2nd-year of graduate school in the UCSF-UC Berkeley Graduate Program in Bioengineering. Thomsen has just joined the Sohn Research Laboratory for her doctoral thesis. The parent grant application pursues the hypothesis that breast cancer risk factors such as age, genetics, and family history integrate through molecular pathways in human mammary epithelial cells (HMECS), resulting in a unique mechanical age in a woman that is distinct from her chronological age. The disparity in mechanical and chronological age measures the susceptibility of a woman’s risk for breast cancer. Under the parent grant, HMEC mechanical properties will be measured using a combination of planar-flow mechano-node-pore sensing (mechano-NPS) and in silico modeling, and mechanical age will be determined through machine learning. Under this Diversity Supplement, Thomsen will develop a new platform—circular mechano-NPS—that will allow her to investigate the mechanical memory of human mammary epithelial cells (HMECs) as they undergo cyclical mechanical dosing. In Aim 1, she will design and prototype the circular mechano-NPS device and test different prototypes using non-malignant MCF10A cells. In Aim 2, she will perform initial mechanical memory experiments with HMEC strains. Ultimately, Thomsen will explore the differences in the mechanical memory of HMECs among young and old women and women who carry who carry germline BRCA1, BRCA2, or PALB2 variants. Mentoring and career development plans for Thomsen are outlined in this supplement, as well as how Thomsen’s appointment promotes diversity within UC Berkeley and the national STEM community. .

摘要 这是R 01 EB 024989的多样性补充，用于泰勒·罗森的职业发展，第一个- 一代大学生谁是进入她的研究生院在UCSF-加州大学伯克利分校研究生院的第二年 在生物工程程序。Bogsen刚刚加入了Sohn研究实验室，完成她的博士论文。的 父母补助金申请追求的假设是，乳腺癌的危险因素，如年龄，遗传学和家庭 在人类乳腺上皮细胞（HMECS）中，历史通过分子途径整合，导致独特的 女性的机械年龄与实际年龄不同。在机械和 实足年龄测量妇女患乳腺癌风险的易感性。根据父母补助金，HMEC 机械性能将使用平面流动机械节点孔隙感测的组合来测量 （机械-机械）和计算机建模，并且机械年龄将通过机器学习来确定。下 在这项多样性补充计划中，Bogsen将开发一种新的平台--循环机械--这将使她能够 研究人类乳腺上皮细胞（HMEC）在经历周期性 机械加药。在目标1中，她将设计和原型圆形机械装置，并测试不同的 使用非恶性MCF 10A细胞的原型。在目标2中，她将进行初步的机械记忆实验， HMEC菌株。最后，Albersen将探索HMEC的机械记忆的差异， 年轻和老年妇女以及携带生殖系BRCA 1、BRCA 2或PALB 2变体的妇女。辅导 和职业发展计划的Bullsen概述在这个补充，以及如何Bullsen的 任命促进了加州大学伯克利分校和国家STEM社区的多样性。 .