Non-human primate MHC and KIR Allele Discovery and Typing Technology Development

非人灵长类 MHC 和 KIR 等位基因发现和分型技术开发

• 批准号: 10849594
• 负责人: DAVID O'CONNER, PHD
• 金额: $ 118.61万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2028-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10849594
• 关键词: Alleles; Animals; Biological Assay; Catalogs; Communities; Complementary DNA; Data Analyses; Development; Fc Receptor; Gene Frequency; Genes; Genetic Services; Genomic DNA; Genotype; Goals; Immunoglobulins; Killer Cells; Laboratories; Libraries; MHC Class I Genes; MHC Class II Genes; Macaca fascicularis; Macaca mulatta; National Institute of Allergy and Infectious Disease; Population; Pre-Clinical Model; Receptor Gene; Research; Resolution; Technology; Transplantation; data curation; exome sequencing; gene discovery; genome sequencing; genomic data; genotyping technology; improved; member; nonhuman primate; programs; public database; receptor; technology development; whole genome

The objective of this program is to discover new NHP MHC Class I, MHC Class II, Killer-cell Immunoglobulin like Receptor (KIR), and Fc Receptor (FcR) genes, loci, and alleles; develop genotyping technologies that will remain freely accessible to the NHP research community; provide all sequences generated to the NIAID-designated, publicly available databases. Other important goals are to: i) develop and apply whole genome/whole exome sequencing (WGS, WES respectively) to the MHC, KIR, and FcR regions; and ii) generate and make publicly available supplemental information, including: a) primer and probe definitions used in gene/allele discovery or genotyping; and b) familial relationships of animals used in gene discovery and allele frequency studies. This information will support the development and interpretation of transplantation and other studies in the NHP pre-clinical model

该计划的目的是发现新的NHP MHC I类，MHC II类，杀伤细胞免疫球蛋白样受体（KIR）和Fc受体（FcR）基因，基因座和等位基因;开发基因分型技术，将保持免费访问NHP研究社区;提供所有序列产生的NIAID指定的，公开可用的数据库。其他重要目标是：i）开发和应用全基因组/全外显子组测序（分别为WGS、WES）到MHC、KIR和FcR区域;和ii）产生和公开可获得的补充信息，包括：a）用于基因/等位基因发现或基因分型的引物和探针定义;和B）用于基因发现和等位基因频率研究的动物的家族关系。 这些信息将支持NHP临床前模型中移植和其他研究的发展和解释