GENOMECPA--A TOOL FOR BIOCHEMICAL SCREENING

GENOMECPA——生化筛选工具

• 批准号: 2611446
• 负责人: DANIEL J LEW
• 金额: $ 15.4万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-20 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2611446
• 关键词: fungal antigens; fungal genetics; genome; microorganism culture; peptide library; recombinant proteins; yeasts

A novel idea is presented which combines the information from the yeast genome with available technologies to generate a new research tool called genomeCPA. The basic idea behind genomeCPA is to produce a set of 60 96-well plates in which each well contains a single distinct immobilized protein of known identity from the yeast genome. The set of 60 plates would then constitute a spatially ordered complete protein array of all the proteins in a yeast cell. Any desired biochemical activity assay could then be performed on the entire array, identifying in one step all of the proteins in the cell possessing the desired activity. The specific aim of this application is to prove the feasibility of the genomeCPA concept. If it is feasible, genomeCPA has the potential to be an extremely powerful new tool for identifying proteins with particular biochemical properties. In the long term, its development should speed the pace of biochemical research and open new horizons of technical feasibility for larger scale projects.

提出了一个新的想法，它结合了信息， 酵母基因组与现有的技术，以产生一个新的 一种叫做基因组CPA的研究工具 背后的基本思想 genomeCPA是生产一组60个96孔板，其中每个 孔含有已知的单一不同的固定化蛋白质， 与酵母基因组的同一性 然后，这组60个板将 构成了一个空间有序的完整的蛋白质阵列的所有 酵母细胞中的蛋白质。 任何所需的生化活性测定 然后可以在整个阵列上执行， 使细胞中所有具有所需活性的蛋白质步聚。 本申请的具体目的是证明 基因组CPA概念。 如果可行，基因组CPA具有 有可能成为一个非常强大的新工具， 具有特殊生化特性的蛋白质。 从长远来看， 它的发展将加速生物化学研究的步伐 并为更大规模的技术可行性开辟了新的视野 项目