HNRNP A1 PROTEIN--RNA/RNA ANNEALING AND MRNA PROCESSING

描述：（改编自申请人摘要）。 的总目标 本研究旨在了解hnRNP A1蛋白（A1）和其他 前mRNA包装和加工中密切相关的hnRNP A/B蛋白， 细胞核。 hnRNP A/B蛋白是丰富的核蛋白 在后生动物进化过程中高度保守。 最近的研究 已经证明，这些蛋白质促进快速退火， 互补RNA链，并在剪接位点选择中发挥作用。 这些 活性对于调节基因表达是重要的， 真核生物 与Adrian Krainer博士和Akila Mayeda博士合作， 冷泉港，调查人员将准备一系列的变体表格 为了鉴定A1的各个结构域内的区域， 是前体mRNA选择性剪接的关键。 变体 蛋白质的特征在于它们的RNA结合和RNA-RNA结合。 退火性能 然后，选定的蛋白质将用于研究 A1对位点特异性结合和蛋白促进的 与不同类型的RNA底物退火。 这些研究 对于理解A1与前体mRNA的相互作用以及 核糖核蛋白装配和前mRNA加工中的hnRNP A/B蛋白。 这些研究还将提供对hnRNP A/B蛋白在细胞凋亡中的作用的深入了解。 调节替代加工和加工mRNA的输出， 细胞质

DESCRIPTION: (Adapted from the Applicant's Abstract). The overall goal of this research is to understand the role of hnRNP A1 protein (A1) and other closely related hnRNP A/B proteins in pre-mRNA packaging and processing in the cell nucleus. The hnRNP A/B proteins are abundant, nuclear proteins which are highly conserved throughout metazoan evolution. Recent studies have demonstrated that these proteins promote the rapid annealing of complementary RNA strands, and play a role in splice site selection. These activities are important for the regulation of gene expression in eukaryotes. In collaboration with Drs. Adrian Krainer and Akila Mayeda at Cold Spring Harbor, the investigators will prepare a series of variant forms of A1 in order to identify regions within the individual domains of A1 which are essential for activity in alternative pre-mRNA splicing. Variant proteins will be characterized with respect to their RNA binding and RNA-RNA annealing properties. Selected proteins will then be used to study the requirements of A1 for site-specific binding and protein-facilitated annealing with different types of RNA substrates. These studies are important for understanding the interactions of A1 with pre mRNA and role of hnRNP A/B proteins in ribonucleoprotein assembly and pre-mRNA processing. The studies will also provide insight into the role of hnRNP A/B proteins in the regulation of alternative processing and export of processed mRNAs to the cytoplasm.