HNRNP A1 PROTEIN--RNA/RNA ANNEALING AND MRNA PROCESSING

描述：(改编自申请人的摘要)。的总目标是 本研究旨在了解hnRNP A1蛋白(A1)和其他 紧密相关的hnRNP A/B蛋白在前mRNA包装和加工中的作用 细胞核。HnRNP A/B蛋白是丰富的核蛋白 它们在后生动物进化过程中高度保守。最新研究 已经证明，这些蛋白质促进了细胞的快速退变 互补的RNA链，并在剪接位点选择中发挥作用。这些 活性对基因表达的调节非常重要。 真核生物。与禤浩焯·克雷纳博士和阿基拉·马伊达博士在 冷泉港，调查员将准备一系列变种形式 为了识别A1的各个结构域内的区域， 对于选择性的前信使核糖核酸剪接是必不可少的。变体 蛋白质将根据它们的RNA结合和RNA-RNA来表征 退火性。然后，选定的蛋白质将被用于研究 A1对位点特异性结合和蛋白质促进的要求 用不同类型的RNA底物进行退火。这些研究是 对理解A1与Pre mRNA的相互作用及其作用 HnRNP A/B蛋白在核糖核蛋白组装和前mRNA加工中的作用。 这些研究还将深入了解hnRNP A/B蛋白在 对加工后的mRNAs的替代加工和出口的监管 细胞质。

DESCRIPTION: (Adapted from the Applicant's Abstract). The overall goal of this research is to understand the role of hnRNP A1 protein (A1) and other closely related hnRNP A/B proteins in pre-mRNA packaging and processing in the cell nucleus. The hnRNP A/B proteins are abundant, nuclear proteins which are highly conserved throughout metazoan evolution. Recent studies have demonstrated that these proteins promote the rapid annealing of complementary RNA strands, and play a role in splice site selection. These activities are important for the regulation of gene expression in eukaryotes. In collaboration with Drs. Adrian Krainer and Akila Mayeda at Cold Spring Harbor, the investigators will prepare a series of variant forms of A1 in order to identify regions within the individual domains of A1 which are essential for activity in alternative pre-mRNA splicing. Variant proteins will be characterized with respect to their RNA binding and RNA-RNA annealing properties. Selected proteins will then be used to study the requirements of A1 for site-specific binding and protein-facilitated annealing with different types of RNA substrates. These studies are important for understanding the interactions of A1 with pre mRNA and role of hnRNP A/B proteins in ribonucleoprotein assembly and pre-mRNA processing. The studies will also provide insight into the role of hnRNP A/B proteins in the regulation of alternative processing and export of processed mRNAs to the cytoplasm.