FLUORESCENT ACTIVATED CELL SORTING FOR ENZYME SCREENING

对研究试剂、诊断试剂的需求一直在增加 蛋白质基催化剂的试剂和化学加工工业 具有新奇能力的。目前，这一需求在很大程度上得到了解决 使用从各种培养微生物中提纯的酶， 通常是细菌界和古生物界的成员。然而，因为 只有不到1%的自然产生的微生物在 文化，必须开发替代技术，以充分利用 具有潜在价值的新产品的多样性。我们建议 开发荧光激活细胞分选以实现极高的吞吐量 从未培养生物中筛选重组表达克隆 发现大量独特的、强大的、特定的生物催化剂 用于生产手性合成子的手性合成子的合成 药品、生物中间体和天然产品。 建议的商业应用： 化学加工工业已经开始增长。这些业务的增长 FIELS的限制是由于缺乏在 所需的加工条件。RBI目前提供了许多酶 各种酶类的文库(糖苷酶；酯酶/脂肪酶； 氨基转移酶；磷酸酶)适合于筛选。这个项目将 用来增加从RBI获得的酶的数量 研究人员和工艺工程师可以设计新的方法。

There has been increasing demand in the research reagent, diagnostic reagent and chemical process industries for protein-based catalysts possessing novel capabilities. At present, this need is largely addressed using enzymes purified from a variety of cultivated microorganisms, generally members of the domains Bacteria and Archaea. However, because less than 1% of naturally occurring microorganisms have been grown in culture, alternative techniques must be developed to exploit the full breadth of diversity for potentially valuable new products. We propose to develop fluorescent activated cell sorting for an extreme high throughput screening for recombinant expression clones from uncultivated organisms to discover a large number of unique, robust, specific biocatalysts for the cost effective synthesis of chiral synthons for use in production of pharmaceuticals, biological intermediates and natural products. PROPOSED COMMERCIAL APPLICATION: and chemical process industries has already begun to grow. Growth in these fields is limited by a lack of enzyme activities useful under the processing conditions required. RBI currently provides a number of enzyme libraries for various enzyme classes (Glycosidases; Esterases/Lipases; Aminotransferases; Phosphatases) suitable for screening. This project will serve to increase the number of enzymes available from RBI with which researchers and process engineers can design new methods.