SCREENING OF ANEUPLOIDY IN HUMAN OOCYTES

通过试管受精从女性获得的人类胚胎比例很高 35岁及以上是非整倍体，这在一定程度上解释了 植入与产妇年龄有关。该项目将开发一种 筛选所有非整倍体卵母细胞的有效而安全的方法。 它的临床应用应该会增加妊娠率 试管受精后的比率和非整倍体受孕的减少。极地 对胚胎发育非必要的身体将被活组织检查 每个卵母细胞，用多色FISH分析。研究材料将 来自未受精或体外成熟的M-I卵母细胞的极体。 因为染色质在极体中迅速退化，活组织检查 将开发出可靠的细胞遗传学方法 低风险损害卵母细胞或受精卵的信息。不同 温度和湿度条件以及固色剂溶液 将进行评估，以优化极体固定。最初，只有 导致卵母细胞非整倍体的最主要的染色体将是 评估过了。这些将通过研究相当数量的 卵母细胞。在Il SBIR阶段的提案中，所有的染色体都将 通过使用5种或5种以上荧光染料和 通过图像分析来实现分辨率。 建议的商业应用为所有人提供植入前诊断 极体中的非整倍体将被开发来改善试管受精 通过降低怀上三体婴儿的几率来提高结局， 以及提高怀孕率。大多数试管受精设备都不能完成这项工作 程序，这样纽约和新泽西的艺术学院就可以收到固定的 这些单位的极体用于胚胎移植前的诊断 在那些试管受精中心。

A high proportion of human embryos obtained through IVF from women 35 and older are aneuploid, in part explaining the decrease in implantation with maternal age. This project will develop an efficient and safe method to screen oocytes for all aneuploidies. Its clinical application should result in an increase in pregnancy rates after IVF and a reduction in aneuploid conceptions. Polar bodies, non-essential for embryo development, will be biopsied from each oocyte and analyzed by multicolor FISH. Research material will be polar bodies from unfertilized or in-vitro matured M-I oocytes. Because chromatin degenerates rapidly in polar bodies, a biopsy method will be developed to provide reliable cytogenetic information with low risk of damaging oocytes or zygotes. Different temperature and humidity conditions as well as fixative solutions will be assessed to optimize polar body fixation. Initially, only those chromosomes most responsible for oocyte aneuploidy will be assessed. These will be determined by studying a sizable number of oocytes. In a phase Il SBIR proposal, all chromosomes will be assessed by using combinations of 5 or more fluorochromes and resolution through image analysis. PROPOSED COMMERCIAL APPLICATIONS Preimplantation diagnosis for all aneuploidies in polar bodies would be developed to improve IVF outcome by reducing the chances of conceiving a baby with trisomy, and increasing pregnancy rates. Most IVF units cannot perform this procedure, so The ART Institute of NY & NJ could receive fixed polar bodies from these units for diagnosis before embryo transfer at those IVF centers.