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SCREENING OF ANEUPLOIDY IN HUMAN OOCYTES

人类卵母细胞非整倍性的筛选

基本信息

批准号：
2418067
负责人：
SANTIAGO MUNNE
金额：
$ 10万
依托单位：
ART INSTITUTE OF NEW YORK AND NEW JERSEY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-10 至 1998-03-09
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2418067
关键词：
aneuploidy biopsy cell components chromatin chromosomes clinical research diagnosis design /evaluation egg /ovum evaluation /testing fluorescent in situ hybridization human tissue in vitro fertilization method development

项目摘要

A high proportion of human embryos obtained through IVF from women 35 and older are aneuploid, in part explaining the decrease in implantation with maternal age. This project will develop an efficient and safe method to screen oocytes for all aneuploidies. Its clinical application should result in an increase in pregnancy rates after IVF and a reduction in aneuploid conceptions. Polar bodies, non-essential for embryo development, will be biopsied from each oocyte and analyzed by multicolor FISH. Research material will be polar bodies from unfertilized or in-vitro matured M-I oocytes. Because chromatin degenerates rapidly in polar bodies, a biopsy method will be developed to provide reliable cytogenetic information with low risk of damaging oocytes or zygotes. Different temperature and humidity conditions as well as fixative solutions will be assessed to optimize polar body fixation. Initially, only those chromosomes most responsible for oocyte aneuploidy will be assessed. These will be determined by studying a sizable number of oocytes. In a phase Il SBIR proposal, all chromosomes will be assessed by using combinations of 5 or more fluorochromes and resolution through image analysis. PROPOSED COMMERCIAL APPLICATIONS Preimplantation diagnosis for all aneuploidies in polar bodies would be developed to improve IVF outcome by reducing the chances of conceiving a baby with trisomy, and increasing pregnancy rates. Most IVF units cannot perform this procedure, so The ART Institute of NY & NJ could receive fixed polar bodies from these units for diagnosis before embryo transfer at those IVF centers.

通过IVF从女性获得的人类胚胎比例很高 35岁及以上是非整倍体，部分解释了与母体年龄有关。该项目将开发一个有效和安全的方法筛选所有非整倍体卵母细胞。它的临床应用应导致增加怀孕 IVF后的比率和非整倍体概念的减少。极性对胚胎发育非必需的身体，将从每个卵母细胞并通过多色FISH进行分析。研究材料将是来自未受精或体外成熟的M-I卵母细胞的极体。由于染色质在极体中迅速变性，将开发一种方法，以提供可靠的细胞遗传学卵母细胞或受精卵受损风险低的信息。不同温度和湿度条件以及固定液将进行评估，以优化极体固定。原本只有那些对卵母细胞非整倍体最有责任的染色体将是评估。这些将通过研究大量的卵母细胞在第II阶段SBIR提议中，所有染色体将被通过使用5种或更多种荧光染料的组合进行评估，分辨率通过图像分析。建议的商业应用所有人的植入前诊断极体中的非整倍体将被开发以改善IVF 通过减少怀上三体性婴儿的机会，增加怀孕率。大多数IVF单位不能执行此操作程序，所以纽约和新泽西州的艺术研究所可以得到固定的在胚胎移植前从这些单位中提取极体进行诊断在那些试管婴儿中心