IN VIVO CONFOCAL MICROSCOPE IMAGING OF OCULAR TISSUES

长期目标是开发和销售高灵敏度和 市场上用于眼组织成像的高灵敏度显微镜系统，在 活着。该项目的具体目标是：1.设计、施工和 测试大数值孔径镜片的前后部成像 晶体晶状体上皮细胞和晶状体纤维，活体，2.至 研究高NA、衍射限制透镜系统的可行性， 具有很强的光学切片特性，用于后部成像 视网膜，活体，3.开发接触元件的光学设计， 将增强表面上皮图像的对比度和细节 细胞，以及4.提高光学关键部件的精度 系统使光学切片和分辨率的全部能力 可供研究和临床用户使用。关于白内障的研究 应促进各种药物的形成和影响 高分辨率图像和跟踪对象的能力 时间段。视网膜图像光学切割性强，图像质量高 分辨率将有助于对黄斑、神经、纤维的研究 层、视盘和视网膜细胞移植的研究。最早的 棘阿米巴、真菌等感染性角膜疾病的诊断 是一个持续不断的目标。 建议的商业应用： 用于研究和临床研究晶状体的仪器， 角膜和视网膜。它扩展了裂隙灯的功能 通过其更高的分辨率、光学切片能力和增强 检测名义上透明组织中微弱细节的灵敏度。 角膜疾病的早期诊断是一个特别有前途的应用。

The long term objective is to develop and market high sensitivity and market high sensitivity microscope systems for imaging ocular tissues, in vivo. Specific aims for this project are: 1. to design, construct, and test high numerical aperture lenses for imaging the anterior and posterior crystalline lens epithelial cells and lens fibers, in vivo, 2. To investigate the feasibility of a high NA, diffraction limited lens system, with strong optical sectioning characteristics, for imaging the posterior retina, in vivo, 3. To develop optical designs of contact elements that will enhance the contrast and detail in images of superficial epithelial cells,and 4. To refine the precision of critical components of the optical system so that the full capabilities for optical sectioning and resolution are available to research and clinical users. The study of cataract formation and the influence of various drugs should be facilitated by the high resolution images and the ability to follow subjects over extended time periods. Retinal images with strong optical sectioning and high resolution will be useful for studies of the macula, the nerve, fiber layer, the disc, and research on retinal cell transplantation. The early diagnosis of infectious corneal diseases such as Acanthamoeba and fungus is a continuing goal. Proposed Commercial Applications: An instrument for research and clinical studies of the crystalline lens, the cornea, and the retina. It extends the capabilities of the slit lamp by its increased resolution, optical sectioning capability, and enhanced sensitivity for detection of faint detail in nominally transparent tissues. Early diagnosis of corneal disease is a particularly promising application.