60 HZ MAGNETIC FIELDS AND CELLULAR STRESS RESPONSE

描述：该提案概述了一系列研究， 最先进的体外电磁场暴露设施，系统地， 60 Hz电磁场暴露对培养人应激反应的影响 细胞 60 Hz电磁场对细胞应激反应的潜在诱导作用 将在一系列简单的实验中得到解决， 他们的设计，规格为远程添加应力诱导 在暴露区域内将试剂输送至细胞培养物。 定量 将在一个设盲的实验中比较假暴露与电磁场暴露。 实验设计 将跟踪几个终点以响应EMF 单独暴露和与已知的应激诱导处理组合， 氧化剂或重金属。 将使用免疫测定来检测 诱导热休克蛋白（HSP）表达的四个成员中的每一个 人类HSP的主要家族。 在另一组实验中， 用低水平氧化应激预处理后， 随后是更高的毒性损伤和细胞存活。 的潜在 60 Hz电磁场暴露对应力反应的影响， 这些实验将被确定。 所有这些实验将 用于彻底记录60 Hz EMF诱导应力的能力 在培养的人类细胞的反应，并将有助于评估的程度， 对整个动物的风险。

DESCRIPTION: This proposal outlines a series of studies using a state-of-the-art in vitro EMF exposure facility to examine, systematically, the effects of 60 Hz EMF exposure on the stress response of cultured human cells. The potential induction of the cellular stress response by 60 Hz EMF will be addressed in a simple series of experiments, with, as integral to their design, specifications for the remote addition of stress-inducing reagents to cell cultures within the exposure field. Quantitative comparisons of sham-exposure versus EMF-exposure will be made in a blinded experimental design. Several endpoints will be followed in response to EMF exposure alone and in combination with known stress-inducing treatments by oxidants or heavy metals. Immunological assays will be used to detect the induction of heat shock protein (HSP) expression for members of each of four major families of human HSP. In a separate set of experiments, a brief pre-treatment with a low-level oxidant stress will be followed by a much higher toxic insult and cell survival will be followed. The potential effects of 60 Hz EMF exposure on the stress response to either phase of these experiments will be determined. All together, these experiments will serve to thoroughly document the ability of 60 Hz EMF to induce a stress response in cultured human cells, and will aid in assessing the extent of risk to whole animals.