EXCITATORY AMINO ACID REGULATION OF VASOPRESSIN CELLS

加压素细胞的兴奋性氨基酸调节

• 批准号: 3395161
• 负责人: JAMES N HAYWARD
• 金额: $ 18.62万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-07-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3395161
• 关键词: electron microscopy; embryo /fetus tissue /cell culture; excitatory aminoacid; glutamate receptor; glutamates; hormone regulation /control mechanism; immunocytochemistry; in situ hybridization; innervation; laboratory rat; neuroendocrine system; neurons; peptide hormone biosynthesis; radioimmunoassay; receptor binding; supraoptic nucleus; tissue /cell culture; vasopressins

Our studies will focus on the chemical and physiological factors which control the secretion of vasopressin by magnocellular neurons in the hypothalamic supraoptic nucleus. These factors will be identified, characterized and an attempt will be made to relate these to the osmotic regulation of vasopressin secretion. The use of an in vitro approach with the hypothalamo-neurohypophysial complex (HNC) will give the degree of control necessary to achieve these goals. The HNC contains the entire supraoptic-neuroendocrine final common pathway including the two intact supraoptic nuclei (NSO), the supraoptico-neurohypophysial tract (SOHT) and the neurosecretory terminals of the neural lobe (NL). Every major site suspected of being involved in the osmotic regulation of vasopressin secretion is present in the HNC. The HNC explant retains osmosensitivity in vitro and can approach in vivo responsiveness. Thus, the entire functional pathway is present for the exploration of the functional integration of osmotic stimuli and the transduction of this information at the level of the supraoptic nucleus. We will characterize the release of vasopressin and establish the conditions which allow precise temporal and semi-quantitative analys of responsiveness via the SOHT. Effects of chemical and osmotic stimuli and their interactions will be characterized and will include screening for the effects of various putative neurotransmitters on the release of vasopressin (cholinergic, angiotensin II, norepinephrine, enkephalin, dopamine). In particular, we will focus on the specific contribution of the NSO with particular emphasis on the nicotinic cholinergic system which has been strongly implicated in the control of vasopressin secretion. The localization of putative nicotinic receptors on vasopressin-containing magnocellular neurons in NSO will be examined. Parallel experiments will be run to assess specific properties of these receptors which may contribute to osmotic sensitivity of the NSO. Investigation of the electrophysiological properties of identified cells in the NSO during chemical and osmotic challenges will provide detailed temporal comparison of the physiological responses with the release of vasopressin. Concomitant double- and triple-labeling procedures on dye-marked neurons will be used for cytoarchitectonic reconstruction and electron microscopic analysis of synaptic inputs on immunocytochemically-identified neurons in the NSO. Thus, the HNC explant represents a simple, viable, highly controlled mammalian neural system in which unknown physiological processes express their effects on a well defined final pathway. Ideal neuroendocrine control system.

我们的研究将集中在化学和生理因素上， 控制垂体大细胞神经元的加压素分泌 下丘脑视上核 将确定这些因素， 特征，并试图将这些与渗透 加压素分泌的调节。 使用体外方法， 下丘脑-神经垂体复合体（HNC）将给出 为实现这些目标所需的控制。 HNC包含整个 视上-神经内分泌最终共同通路，包括两个完整的 视上核（NSO）、视上神经垂体束（SOHT）和 神经叶的神经分泌终末。 每个主要网站 被怀疑与加压素的渗透调节有关 分泌存在于HNC中。 HNC外植体保留了组织敏感性 并可接近体内反应性。 因此，整个 功能途径是目前的探索功能 渗透刺激的整合和这种信息的转导， 视上核的水平 我们将描述释放 加压素，并建立条件，允许精确的时间和 通过SOHT的反应性的半定量分析。 的影响 化学和渗透刺激及其相互作用的特点 并将包括筛选各种假定的 神经递质对血管加压素（胆碱能，血管紧张素 II、去甲肾上腺素、脑啡肽、多巴胺）。 特别是，我们将重点关注 各国统计局的具体贡献，特别强调 烟碱胆碱能系统，这已强烈牵连在 加压素分泌的控制。 假定的烟碱的定位 在NSO中含有加压素的大细胞神经元上的受体将被 考察 将进行平行实验以评估特定性质 这些受体可能有助于NSO的渗透敏感性。 对已鉴定细胞的电生理特性的研究 在化学和渗透挑战期间，国家统计局将提供详细的 释放的生理反应的时间比较 加压素 伴随的双标记和三标记程序 染料标记的神经元将用于细胞结构重建， 突触输入的电子显微镜分析 免疫细胞化学鉴定的NSO中的神经元。 因此，HNC外植体 代表了一个简单的，可行的，高度控制的哺乳动物神经系统， 哪些未知的生理过程会对油井产生影响 确定最终路径。 理想的神经内分泌控制系统。