NIDDK/Baylor Biotech Center

描述(由申请人提供)： 制造微阵列的新方法和新的检测系统已经 是最近在工业和学术环境中开发的，以便 进行新的基因发现，分析表达模式 处于发育和病理状态。总的来说，这些技术是 目前通过商业企业获得的价格非常昂贵，而且 超出以小型大学为基础的研究实验室的预算。这个 这项提议的总体目标是发展基础设施，使NIDDK 资助研究人员获得基因表达微阵列技术。贝勒 医学院(BCM)在发展方面取得了良好的开端 用于基因表达分析的微阵列技术。NIDDK/贝勒 生物技术中心将为我们提供机会建造最多的 目前可用于阵列和开发独特的、 组织特定的序列集，将服务于更特殊的需求 NIDDK调查人员。制作和分析cDNA微阵列的专业知识 存在于贝勒。然而，贝勒工厂目前的产能是 有限，不能有效满足调查人员的需求。这项建议 将满足NIDDK调查人员的要求，提供专门的 共享资源核心内的工作人员将实现以下研究目标 这群调查人员。NIDDK/BCM指导委员会 生物技术中心将协调微阵列生产和 分析。该方案的目标包括构建高密度的cdna微阵列。 密度，使得能够同时测量基因组的主要部分 一个单一的反应。我们将开发表示选定子集的自定义数组 中所表达的基因的Unigene cDNA文库 特定的器官/组织，以加快芯片制备并降低成本。我们 将探索提高微阵列反应灵敏度的策略。 我们将整合生物信息学和生物统计学对微阵列的支持 分析。我们将开发共享数据、试剂和程序的方法 贝勒社区和全国都希望在这方面取得进展 技术我们将为教师、博士后研究员和 贝勒医学院的学生在个人实验室里 由NIDDK以及区域调查人员提供资金。我们会招揽， 选择并支持将受益于使用微阵列的试点项目 基因表达图谱。最后，我们将建立一个管理结构 这将支持NIDDK/BCM生物技术中心和 将利用这一设施的NIDDK调查人员。

DESCRIPTION(provided by applicant): New methodologies for fabricating microarrays and novel detection systems have been developed recently in both industrial and academic settings in order to carry out new gene discovery, analysis of expression patterns during development and in pathological states. In general, the technologies that are currently available through the commercial ventures are very expensive, and beyond the reach of small university based research laboratory budgets. The overall goal of this proposal is to develop the infrastructure to enable NIDDK funded investigators to access gene expression microarray technologies. Baylor College of Medicine (BCM) has made an excellent beginning in the development of microarray technologies for gene expression analysis. An NIDDK/Baylor Biotechnology Center would afford us the opportunity to build the most comprehensive set of genes currently available for array and to develop unique, tissue specific sets of sequences that will serve more specialized needs of NIDDK investigators. The expertise to make cDNA microarrays and to analyze them exists at Baylor. However, the current capacity of the facilities at Baylor are limited and cannot effectively meet the needs of investigators. This proposal will address the requirements of NIDDK investigators by providing dedicated staff within shared resource cores who will carry forward the research aims of this group of investigators. The Steering Committee of the NIDDK/BCM Biotechnology Center will coordinate the processes of microarray production and analysis. The aims of this proposal include building cDNA microarrays of high density, enabling simultaneous measurement of a major portion of the genome in a single reaction. We will develop custom arrays representing a selected subset of the Unigene cDNA libraries that correspond to genes expressed in a particular organ/tissue in order to speed chip preparation and reduce cost. We will explore strategies for increasing sensitivity of the microarray reaction. We will integrate bioinformatic and biostatistical support for microarray analysis. We will develop means of sharing data, reagents and procedures within the Baylor community and nationally to enhance progress in this developing technology. We will provide training to faculty, postdoctoral fellows, and students within Baylor College of Medicine in the laboratories of individuals funded by the NIDDK as well as to investigators regionally. We will solicit, select and support pilot projects that would benefit from the use of microarray gene expression profiling. Finally we will develop an administrative structure that will support the activities of the NIDDK/BCM Biotechnology Center and the NIDDK investigators that will utilize this facility.